Fig 2: HSD17B6 inhibits the cell migration and invasion of LUAD cells.A The effect of HSD17B6 overexpression on cell migration of H1299 and H1975, as determined using wound-healing assays. B, C The effect of HSD17B6 on cell migration and invasion of H1299 and H1975, as determined using transwell assay. D Protein levels of E-cadherin, N-cadherin, Vimentin, Snail, MMP2, and MMP9 were assessed by western blotting after HSD17B6 overexpression. OE-NC, negative control for overexpression; OE-HSD17B6, HSD17B6 overexpression. E–P Correlation analysis of HSD17B6 expression and expression of invasion-related genes (CDH1, CDH2, SNAI1, and MMP9) in TCGA LUAD, GSE68465, and GSE72094 datasets.

Fig 3: Catalytic antibodies mediated cardiomyocyte cohesion in AC patients. In normal healthy individuals, the cadherin family proteins, desmoglein 2 (DSG2) and N-cadherin (N-CAD) proteins are properly localized to the ICD via the plaque proteins DSP and PKP2 and armadillo proteins PG, a-catenin (a), ß-catenin (ß) and YAP (Y), respectively. In AC patients, having mutations in either DSP or PKP2 genes (the corresponding proteins are represented with dashed boxes), presence of catalytic antibodies (CA) that can cleave DSG2 or N-CAD could destabilize the cadherin-mediated adhesion (1), which could lead to the activation of p38MAPK (2), which further can lead to desmosome destabilization. Cleavage of cadherin proteins combined with p38MAPK activation could eventually result in reduced cardiomyocyte cohesion (3) and thereby a reduction in the mechanical strengths of the cardiomyocyte. Cartoon created with BioRender.com

Fig 4: Biological functions of circAGTPBP1 in PTC(A–F) qRT-PCR analysis of circAGTPBP1 and AGTPBP1 mRNA in TPC-1 and B-CPAP cell lines treated with three designed siRNAs. Proliferation ability of TPC-1 and B-CPAP cells transfected with si-circAGTPBP1 or NC as determined by CCK-8 (B), colony formation (C) and EdU assays (D). Scale bar: 100 µm. The migratory and invasive capabilities of TPC-1 and B-CPAP cells transfected with si-circAGTPBP1 or NC as assessed by wound healing (E, Scale bar: 20 µm) and Transwell assays (F).(G) Immunofluorescence of E-cad and N-cad in TPC-1 and B-CPAP cells transfected with si-circAGTPBP1 or NC. Scale bar: 20 µm.(H) Morphological changes in TPC-1 cells transfected with si-circAGTPBP1 or NC. Scale bar: 10 µm. Each experiment was repeated three times with triplicates. PTC, papillary thyroid cancer; DAPI, 4,6-Diamidine-2-phenylindole; qRT-PCR, quantitative real-time polymerase chain reaction; NC, normal control; CCK-8, cell counting kit-8. Data are shown as the mean ± SD, **p < 0.01, ***p < 0.001. Unpaired t test for (B), (C), (D), (E), and (F); one-way analysis of variance (ANOVA) test for (A).

Fig 5: TRIM29 inhibits EMT, migration, invasion, and metastasis of ESCC cells.A, B GSEA analysis shows a negative correlation between TRIM29 mRNA levels and “HALLMARK EPITHELIAL MESENCHYMAL TRANSITION” signature in TCGA ESCC and GSE21293. C Western blot analysis of Snail, Vimentin, N-cadherin, E-cadherin, MMP9, and MMP2 protein levels after TRIM29 overexpression in KYSE30, and TRIM29 knockdown in KYSE410/KYSE450 cells. D, E Scatterplot of mRNA expression correlation between TRIM29 and CDH1/CDH2/VIM/SNAI1 in TCGA ESCC and GSE21293. F Scatterplot of protein expression correlation between TRIM29 and CDH1/CDH2/VIM in Liu2021 dataset. G, H Scatterplot of mRNA expression correlation between TRIM29 and MMP2/MMP9 in TCGA ESCC and GSE21293. I Scatterplot of protein expression correlation between TRIM29 and MMP2/MMP9 in Liu2021 dataset. J–L The effect of TRIM29 expression on cell migration of KYSE30, KYSE410, and KYSE450 was evaluated by wound-healing assay. M–O The effect of TRIM29 expression on cell invasion of KYSE30, KYSE410, and KYSE450 was evaluated by matrigel-transwell assay. P KYSE30 or TRIM29-overexpressing KYSE30 cells were injected into the tail vein of SCID/Beige mice (5 mice in each group). Left: the lungs were harvested 12 weeks post-injection, and the surface metastatic nodules were counted (red arrows: metastatic nodules on lung surface). Right: quantitative analysis of metastatic nodes on lung surface. *p < 0.05. Q Representative images of lung sections stained with H&E. OE overexpression, KD knockdown.