Fig 2: Effect of CSF1-Fc on PBMCs. Pigs (8-wk-old males and females) were injected with PBS or 0.75 mg/kg CSF1-Fc for 3 days prior to euthanasia on day 4. Blood was collected as described in materials and methods. PBMCs were analyzed via flow cytometry for expression of CD16 (A), CD172a (B), CD3 gated on the lymphocyte population exclusively (C), or CD163/CD14 (D) with exclusion of dead cells using SYTOX blue. Representative flow cytometry plots are shown. Graphs show means ± SE. **P < 0.01, ***P < 0.001 by t-test; n = 4–5 pigs per treatment.

Fig 3: CD163+ rates on PAM cells and IM cells isolated from healthy pigs. Gray area represents the surface CD163 expression levels in PAM cells (A) and IM cells (B) and the white area represents the isotype control. **P<0.01.

Fig 4: Effect of CSF1-Fc on bone marrow (BM) cells. Pigs (8-wk-old males and females) were injected with PBS or 0.75 mg/kg CSF1-Fc for 3 days prior to euthanasia on day 4. BM from ribs was collected as described in materials and methods. BM cells were analyzed via flow cytometry for expression of CD14 (A), CD163 (B), CD169 (C), or cKit (D) with exclusion of dead cells using SYTOX blue. Representative flow cytometry plots are shown. Graphs show means ± SE. **P < 0.01, ****P < 0.0001 by t-test; n = 4–5 pigs per treatment.

Fig 5: Effect of a short course of CSF1-Fc in weaning pigs. Pigs (4-wk-old males and females) were injected with PBS or 1 mg/kg CSF1-Fc for 2 days prior to euthanasia on day 3. Graphs show means ± SE. *P < 0.05, **P < 0.01, ***P < 0.001 by t-test; n = 5 pigs per treatment. A: blood was collected into EDTA tubes postmortem and complete blood count assessment was performed. Graphs show liver weight/body weight ratio, spleen weight/body weight ratio, and monocyte number. B: BM from ribs was collected as described in materials and methods. BM cells were analyzed via flow cytometry for expression of CD163 with exclusion of dead cells using SYTOX blue. Representative flow cytometry plots are shown. C: formalin-fixed liver and spleen tissue was prepared and stained for CD163. Representative images are shown.