CD45 Antibody Pacific Orange MHCD4530 from Thermo Fisher Scientific

Product Specs
ItemCD45 Antibody Pacific Orange
CompanyThermo Fisher Scientific
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberMHCD4530
Regulatory StatusASR, IVD - analyte specific reagent
ApplicationsFlow Cytometry
CloneHI30
HostMouse
ClonalityMonoclonal
TargetCD45
ReactivityHuman
Conjugate/TagPacific Orange™
Absorbance / Excitation405 nm, 551 nm
NCBI Full Gene Nameprotein tyrosine phosphatase receptor type C
NCBI Gene AliasesB220, CD45, CD45R, GP180, IMD105, L-CA, LCA, LY5, T200
Quantity500 µL
Brand/TrademarkMolecular Probes®
FormatPBS with 4mg/mL BSA and 0.1% sodium azide
IsotypeIgG1
ImmunogenCD45 antigen
Storage Temperature4° C, store in dark
Antibody TypePrimary
Storage4° C, store in dark

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

Company Profile

Website: www.thermofisher.com

(1) Purification of primary human placental leukocytes to study maternal-fetal interactionsSTAR ProtocolsJune 1, 2023Nadia M. Ikumi, Zachary Koenig, Shweta Mahajan, Clive M. Gray, Tamara TilburgsRESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, CD45 - Pacific Orange; HI30 (1:50),Invitrogen,RRID:AB_10376143; Cat #MHCD4530 CD14 - PerCP; HCD14 (1:50),BioLegend,RRID:AB_2564058; Cat #301847 CD56 - PE; HCD56 (1:50),BioLegend,RRID:AB_604093;

(2) Improving NK cell function in multiple myeloma with NKTR-255, a novel polymer-conjugated human IL-15Blood advancesJanuary 10, 2023Fernandez RA, Mayoral JE, Pierre-Louis L, Yao Y, Xu Y, Mu S, Martinez-Lopez J, Primo D, Miyazaki T, Prabhala R, et al.Cat.#310928), anti-CD3-Pacific Blue (BD Biosciences Cat.#558117), anti-CD45-Pacific Orange (Invitrogen, Cat.#MHCD4530), anti-CD38-APC (Biolegend, Cat.#356606), and anti-CD56-APC/Cy7 (Biolegend, Cat.#318332). In vivo studies All

(3) Effect of omega-3 on HLA-DR expression by conjunctival cells and tear cytokine concentrations in the Dry Eye Assessment and Management (DREAM) StudyEye & Contact Lens: Science & Clinical PracticeSeptember 1, 2022Neeta S. Roy, Yinxi Yu, Gui-Shuang Ying, Maureen G. Maguire, Penny A. Asbell, for the DREAM Study GroupClone EBA-1), pacific orange labelled anti-CD45 (pan-white blood cell marker) antibody (Invitrogen, Cat#MHCD4530, CloneH130). Flow cytometer analysis on a BD LSR Fortessa cell analyzer of the stained cells was performed

(4) Platelet-Depletion of Whole Blood Reveals That Platelets Potentiate the Release of IL-8 From Leukocytes Into Plasma in a Thrombin-Dependent MannerFrontiers in ImmunologyApril 4, 2022Huy Quang Quach, Christina Johnson, Karin Ekholt, Rakibul Islam, Tom Eirik Mollnes, Per H. Nilssonof the stop solution, the leukocytes were stained with antibodies: CD45-Pacific Orange (catalog no. MHCD4530, Invitrogen™), CD14-PerCP (catalog no. 340585, BD Biosciences), CD15-V450 (catalog no. 48-0158-42, Invitrogen™),

(5) Enhancer Hijacking Drives Oncogenic BCL11B Expression in Lineage-Ambiguous Stem Cell LeukemiaCancer discoveryNovember 1, 2021Montefiori LE, Bendig S, Gu Z, Chen X, Pölönen P, Ma X, Murison A, Zeng A, Garcia-Prat L, Dickerson K, et al.10510), a cocktail of cell surface marker antibodies, containing CD45-Pac Orange (5 μL; Life Technologies MHCD4530), CD235a-PE (5 μL; Beckman Coulter IM2211U), CD34-PerCP-Cy5.5 (20 μL; BD Biosciences 347213), CD33-PE-Cy7

(6) Integration of T helper and BCR signals governs enhanced plasma cell differentiation of memory B cells by regulation of CD45 phosphatase activityCell reportsAugust 10, 2021Szodoray P, Andersen TK, Heinzelbecker J, Imbery JF, Huszthy PC, Stanford SM, Bogen B, Landsverk OB, Bottini N, Tveita A, et al.PharMingen,Cat# 740796; RRID:AB_2740459 PO Mouse monoclonal anti human CD45 (clone HI30),ThermoFisher,Cat# MHCD4530; RRID:AB_10376143 Alexa Fluor 647Mouse monoclonal anti human Galectin-1 (clone GAL1/1831),Novus Biologicals,Cat#

(7) Loss of Optineurin Drives Cancer Immune Evasion via Palmitoylation-Dependent IFNGR1 Lysosomal Sorting and DegradationCancer discoveryJuly 1, 2021Du W, Hua F, Li X, Zhang J, Li S, Wang W, Zhou J, Wang W, Liao P, Yan Y, et al.optineurin detection, we prepared by staining with anti-optineurin (sc-166576 AF488, Santa Cruz), anti-CD45 (MHCD4530, ThermoFisher), anti-CD3 (562280, BD Biosciences), anti-CD8 (555368, BD Biosciences), anti-CD4 (562424,

(8) Comparison of Two Strategies to Generate Antigen-Specific Human Monoclonal Antibodies: Which Method to Choose for Which Purpose?Frontiers in immunologyJanuary 1, 2021Ehlers AM, den Hartog Jager CF, Kardol-Hoefnagel T, Katsburg MMD, Knulst AC, Otten HG.US Antibodies Antibodies for Single Cell Sorting CD45-Pacific Orange, clone HI30, final dilution 1:40, MHCD4530, ThermoFisher Scientific, Waltham, Massachusetts, USCD3-Pacific Blue, clone UCHT1, final dilution 1:160,

(9) Optimization and testing of dried antibody tube: The EuroFlow LST and PIDOT tubes as examplesJournal of immunological methodsDecember 1, 2019van der Velden VHJ, Flores-Montero J, Perez-Andres M, Martin-Ayuso M, Crespo O, Blanco E, Kalina T, Philippé J, Bonroy C, de Bie M, et al.

(10) Specific Activation In Vivo of HIV-1 by a Bromodomain Inhibitor from Monocytic Cells in Humanized Mice under Antiretroviral TherapyJournal of virologyJune 15, 2019Li G, Zhang Z, Reszka-Blanco N, Li F, Chi L, Ma J, Jeffrey J, Cheng L, Su L.were purchased from Biolegend; Paciﬁc orange-conjugated anti-mouse CD45 (clone HI30, catalog number MHCD4530), PE-TR-conjugated CD8 (clone 3B5, catalog number MHCD0817), and a LIVE/DEAD ﬁxable aqua dead cell stain

(11) Report of the First National Consensus Meeting for Acute Leukemias ImmunophenotypingGaceta de M�xicoJune 7, 2019Lourdes Arriaga-Pizano, Dalia Ramírez-Ramírez, Jessica Prieto-Chávez, Rosana Pelayo, Alejandro Ruiz-Argüellesphotocopying without the prior written permission o f the publisher. © Permanyer 2019 Marker MHCD4530 CD45 PE‑Cy7 HI30 BioLegend 304016 CD64 FITC 10.1 BioLegend 305006 CD71 FITC M‑A712 BD Biosciences

(12) Scalable high-throughput acoustophoresis in arrayed plastic microchannelsBiomicrofluidicsMay 1, 2019Dubay R, Lissandrello C, Swierk P, Moore N, Doty D, Fiering J.

(13) Influence of nasal polyp tissue on the differentiation and activation of T lymphocytes in a co-culture systemBiomedical reportsFebruary 1, 2019Ickrath P, Scherzad A, Kleinsasser N, Ginzkey C, Hagen R, Hackenberg S.factor-β. Fluorescence‑activated cell sorting (FACS). The following antibodies were used: Anti‑CD45 Pacific Orange (1:300; MHCD4530; Thermo Fisher Scientific, Inc.), anti‑CD3 phycoerythrin (PE)‑Cyanine (Cy)7 (1:300; 300420) anti‑CD4

(14) [Reporte de la Primera Reunión Nacional de Consenso para la Inmunofenotipificación de Leucemias Agudas]Gaceta medica de MexicoJanuary 1, 2019Arriaga-Pizano L, Ramírez-Ramírez D, Prieto-Chávez J, Pelayo R, Ruiz-Argüelles A, Ruiz-Delgado GJ, Marín y López RA.reproducirse ni fotocopiarse ninguna parte de esta publicación. © Permanyer 2019 Marcador 304038 MHCD4530 CD45 PE‑Cy7 HI30 BioLegend 304016 CD64 FITC 10.1 BioLegend 305006 CD71 FITC M‑A712 BD Biosciences

(15) Maturation-associated gene expression profiles during normal human bone marrow erythropoiesisCell death discoveryJanuary 1, 2019Mello FV, Land MGP, Costa ES, Teodósio C, Sanchez ML, Bárcena P, Peres RT, Pedreira CE, Alves LR, Orfao A.

(16) Accumulation of CD69+ tissue‑resident memory T cells in the nasal polyps of patients with chronic rhinosinusitisInternational journal of molecular medicineAugust 1, 2018Ickrath P, Kleinsasser N, Ding X, Ginzkey C, Beyersdorf N, Hagen R, Kerkau T, Hackenberg S.medium. Fluorescence‑activated cell sorting. The following antibodies were used: Anti‑CD45 Pacific Orange (1:300; MHCD4530; Thermo Fisher Scientific Inc., Waltham, MA, USA), anti‑CD3 phycoerythrin (PE)‑Cy7 (1:300; 300420;)

(17) Immunophenotyping and transcriptional profiling of in vitro cultured human adipose tissue derived stem cellsScientific reportsJuly 27, 2018Mieczkowska A, Schumacher A, Filipowicz N, Wardowska A, Zieliński M, Madanecki P, Nowicka E, Langa P, Deptuła M, Zieliński J, et al.

(18) Targeting of colony-stimulating factor 1 receptor (CSF1R) in the CLL microenvironment yields antineoplastic activity in primary patient samplesOncotargetMay 15, 2018Edwards V DK, Sweeney DT, Ho H, Eide CA, Rofelty A, Agarwal A, Liu SQ, Danilov AV, Lee P, Chantry D, et al.CD19-V450 (#644492; BD Biosciences), CD33-PerCP-Cy-5.5 (#341640; BD Biosciences), CD45Pacific-Orange (#MHCD4530; Invitrogen, Carlsbad, CA), CD64-PE (#558592; BD Biosciences), and CSF1RAPC (#347306; BioLegend, San

(19) Intrinsic Immunity Shapes Viral Resistance of Stem CellsCellJanuary 25, 2018Wu X, Dao Thi VL, Huang Y, Billerbeck E, Saha D, Hoffmann HH, Wang Y, Silva LAV, Sarbanes S, Sun T, et al.Biosciences,Cat# 103122 RRID:AB_493531 Pacific Orange Mouse anti-human CD45,Life Technologies,Cat# MHCD4530 RRID:AB_10376143 APC Mouse anti-human CD19,BD Biosciences,Cat# 555415 RRID:AB_398597 PerCP-Cy5.5 Mouse

(20) Immunodeficiency in Bloom's SyndromeJournal of clinical immunologyJanuary 1, 2018Schoenaker MHD, Henriet SS, Zonderland J, van Deuren M, Pan-Hammarström Q, Posthumus-van Sluijs SJ, Pico-Knijnenburg I, Weemaes CMR, IJspeert H.

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CD45 Antibody Pacific Orange from Thermo Fisher Scientific

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CD45 Antibody Pacific Orange from Thermo Fisher Scientific

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