Fig 2: CD56dim and CD56neg Subset Phenotypic Analysis, Related to Figure 3(A and B) Summary boxplots of expression of NKG2A, NKG2C, iKIR (cocktail of antibodies against KIR2DL1/S5, KIR2DL2/L3/S2, KIR3DL2, KIR3DL1), CD85j, CD57, FceRI-?, PLZF and Siglec7 on CD56dim NK cells and (B) on the CD56neg NK cell subset in bnAb (red; n = 22), control (blue; n = 19) and HIV seronegative individuals (black; n = 22). Each symbol represents data from an individual subject and the box-and-whisker plots show the median, quartiles and range. *p < 0.05, **p < 0.01, ***p < 0.001. P values corrected for MCA and viral load.

Fig 3: Increased Frequencies of Mature and Adaptive-like NK Cells in bnAb Subjects(A–H) Boxplots of the percentage of total NK cells expressing NKG2A (A), NKG2C (B), iKIR (cocktail of antibodies against KIR2DL1/S5, KIR2DL2/L3/S2, KIR3DL2, KIR3DL1) (C), CD85j (D), CD57 (E), Siglec7 (F), FceRI-? (G), and PLZF (H) in bnAb (red; n = 22), control (blue; n = 19) and HIV seronegative individuals (black; n = 22). Each symbol represents data from an individual subject and the box-and-whisker plots show the median, quartiles, and range. *p < 0.05, **p < 0.01, ***p < 0.001. p values are corrected for multiple comparisons analysis and viral load.(I) SPICE (simplified presentation of incredibly complex evaluations) analysis of CD57, FceRI-?, PLZF, and Siglec7 receptor combinations on total NK cells in HIV seronegative individuals (n = 22), control (n = 19) and bnAb (n = 22) subjects. SPICE pie charts are shown for seronegative, control and bnAb groups and for individuals with the lowest RAB11FIP5 mRNA (bottom 5; blue; n = 5) in the control group and highest levels of expression of RAB11FIP5 mRNA in total PBMC (top 5; red; n = 5) in the bnAb group. SPICE bar charts depict the proportion of total NK cells expressing the indicated receptor combinations in the top 5 RAB11FIP5 subjects in the bnAb group (red bars) and bottom 5 RAB11FIP5 subjects in the control group (blue bars). Student’s t test was used to compare samples in SPICE. + p < 0.0001.See also Figures S4 and S5.