Fig 1: Protein expression and relative density of Kv4.3 and KChiP2 subunits determined by western blotting and immunocytochemistry in left ventricular samples from SED and TRN dogs.(A and B) Relative protein expression of Kv4.3 and KChiP2 subunits determined by western blotting in left ventricular samples of SED (n=12) and TRN (n=12) dogs, respectively. (C) Representative image of Kv4.3 and KChIP2 bands and their corresponding loading controls (GAPDH). (D and E) Relative densities of Kv4.3 and KChiP2 protein immunolabeling obtained from SED (n=30 cells/6 dogs) and TRN (n=30 cells/6 dogs) cardiomyocytes. (F) Representative immunofluorescence images of canine cardiomyocytes with Kv4.3 and KChiP2 immunolabeling. The ‘n’ numbers refer to the number of dogs (A and B) or the number of cells followed by the number of dogs from which the cells were obtained (D and E). Data are expressed as mean ± SEM. Blue dots represent individual data. Figure 6—source data 1.Relative protein expression of Kv4.3 subunit determined by western blotting in sedentary and trained dogs. Figure 6—source data 2.Relative protein expression of KChiP2 subunit determined by western blotting in sedentary and trained dogs. Figure 6—source data 3.Relative density of Kv4.3 subunit determined by immunocytochemistry in sedentary and trained dogs. Figure 6—source data 4.Relative density of KChiP2 subunit determined by immunocytochemistry in sedentary and trained dogs. Figure 6—source data 5.Original unedited membranes of western blots with the relevant bands clearly labeled. Figure 6—source data 6.Original files of the full raw unedited membranes of western blots.