Fig 2: BEL impedes NR4A1 from the nucleus to cytoplasmic localization (A) Effects of BEL, SB203580 and LY2157299 on the expression of NR4A1 and P-NR4A1 induced by TGF-ß1 using immunofluorescence staining. Cell nuclei was counterstained in blue with DAPI (B) Expression of NR4A1 and the levels of P-NR4A1 in total protein (T-NR4A1, T-P-NR4A1) by western blotting. Bar graphs show fold changes of P-NR4A1/NR4A1 (n = 3) (C) Expression of NR4A1 and the levels of P-NR4A1 in cytoplasmic protein (C-NR4A1, C-P-NR4A1) by western blotting. Bar graphs show fold changes of P-NR4A1/NR4A1 (n = 3) (D) Expression of NR4A1 and the levels of P-NR4A1 in nuclear protein (N-NR4A1, N-P-NR4A1) by western blotting. Bar graphs show fold changes of NR4A1/H3 (n = 3). Data were shown as mean ± SEM. **p < 0.01 vs. control, # p < 0.05 vs. TGF-ß1, ## p < 0.01 vs. TGF-ß1.