Fig 1: OX40L on ILC2s Orchestrates Adaptive Type 2 Immunity to Allergens(A–D) Mice of the specified genotypes were treated with PBS or papain (Pap) (i.n., day 0 and 1), followed by quantification of lung Th2, GATA3+ Treg, and GATA3- Treg cells on day 5 (A–C). Whole lung cell suspensions were re-stimulated with PMA and ionomycin, followed by quantification of IL-13+ Th2 cells by intracellular staining (D).(E–G) Mice were treated with papain on days 0, 1, 14, and 21, followed by quantification on day 24 of lung eosinophils (E), and detection (F) and quantification (G) of RELMa+ M2 alveolar (CD45+SiglecF+CD11c+CD11b-F4/80+) macrophages (MF).(H and I) Mice were treated with A. alternata (A.alt) (i.n., days 0 and 1), followed by quantification on day 9 of lung Th2, GATA3+ Treg, and GATA3- Treg cells (H) and serum IgE concentration (I).Bar graphs indicate mean (±SEM). (A)–(D), ANOVA, two repeat experiments; (E), ANOVA, three repeat experiments; (F) and (G), ANOVA, two repeat experiments, representative gate shown in (F); (H) and (I), ANOVA, two repeat experiments. **p = 0.01, ***p = 0.001, ****p = 0.0001. See also Figure S7.