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Pacific Blue(TM) Annexin V
Pacific Blue(TM) Annexin V from BioLegend
(1)
Write a review
Citations:
(59)
Supplier Page
Supplier Page from
BioLegend for
Pacific Blue(TM) Annexin V
Description
Pacific Blue(TM) Annexin V
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Pacific Blue(TM) Annexin V
Company
BioLegend
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
640917
Quantity
25 tests
Applications
Flow Cytometry (FC)
Conjugate/Tag
Pacific Blue
NCBI Gene Aliases
ANX5, CPB-I, ENX2, HEL-S-7, PP4, RPRGL3, VAC-alph
NCBI Full Gene Name
annexin A5
Format
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Target
Annexin V
Species
Human, Mouse, Rat
Add to Compare List
BioLegend
8999 BioLegend Way
San Diego
,
California
92121
United States
Phone
: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587
Company Profile
Website:
biolegend.com
Citations (59)
(21)
Pharmacological Activation of Pyruvate Kinase M2 Inhibits CD4
+
T Cell Pathogenicity and Suppresses Autoimmunity
Cell metabolism
February 4, 2020
Angiari S, Runtsch MC, Sutton CE, Palsson-McDermott EM, Kelly B, Rana N, Kane H, Papadopoulou G, Pearce EL, Mills KHG,
et al
.
K-12049-D50
Propidium Iodide
,
Invitrogen
,Cat #:
P1304MP
; CAS: 25535-16-4 Pacific Blue™ Annexin V,
BioLegend
,Cat #:
640918
PMA,
Sigma Aldrich
,Cat #:
P8139
; CAS:16561-29-8 Ionomycin,
Sigma Aldrich
,Cat #:
I0634
; CAS: 56092-82-1
Brefeldin A
(22)
Mitochondrial DNA Stress Signalling Protects the Nuclear Genome
Nature metabolism
December 1, 2019
Wu Z, Oeck S, West AP, Mangalhara KC, Sainz AG, Newman LE, Zhang XO, Wu L, Yan Q, Bosenberg M,
et al
.
(Dako NA Inc, S3023),
annexin V binding buffer
(
Biolegend
, #42220), Pacific Blue annexin V (
Biolegend
, #
640918
) and propidium iodide (MP Biomedicals, #195458), crystal
violet
(Fisher Scientific, #C581–25), PicoGreen
(23)
A Kinase Inhibitor with Anti-Pim Kinase Activity is a Potent and Selective Cytotoxic Agent Toward Acute Myeloid Leukemia
Molecular cancer therapeutics
March 1, 2019
Bjørnstad R, Aesoy R, Bruserud Ø, Brenner AK, Giraud F, Dowling TH, Gausdal G, Moreau P, Døskeland SO, Anizon F,
et al
.
(24)
N-Cadherin-Expressing Bone and Marrow Stromal Progenitor Cells Maintain Reserve Hematopoietic Stem Cells
Cell reports
January 15, 2019
Zhao M, Tao F, Venkatraman A, Li Z, Smith SE, Unruh J, Chen S, Ward C, Qian P, Perry JM,
et al
.
Streptavidin Alexa Fluor 405 conjugate
(Invitrogen). For apoptosis analysis, Pacific Blue Annexin V (
640918
,
BioLegend
) was used. For BrdU analysis,
APC BrdU Flow Kit
(51–9000019BK
BD
-PharMingen) was used.
Cell sorting
(25)
IL22 Inhibits Epithelial Stem Cell Expansion in an Ileal Organoid Model
Cellular and molecular gastroenterology and hepatology
January 1, 2019
Zwarycz B, Gracz AD, Rivera KR, Williamson IA, Samsa LA, Starmer J, Daniele MA, Salter-Cid L, Zhao Q, Magness ST.
(
1:100
, FAB4291P; R&D Systems). Immediately before FACS analysis of live cells, AnnexinV Pac-Blue (
1:100
,
640918
;
Biolegend
) was used for live/dead discrimination. All FACS
and flow cytometry
experiments were performed
(26)
Cyclosporine H Overcomes Innate Immune Restrictions to Improve Lentiviral Transduction and Gene Editing In Human Hematopoietic Stem Cells
Cell stem cell
December 6, 2018
Petrillo C, Thorne LG, Unali G, Schiroli G, Giordano AMS, Piras F, Cuccovillo I, Petit SJ, Ahsan F, Noursadeghi M,
et al
.
Commercial
Assays
,Critical Commercial
Assays
,, Annexin V Apoptosis Detection Kit I,
BioLegend
,Cat#
640918
; RRID:AB_1279044 Cell Proliferation Dye eFluor® 670,
Thermo Fisher Scientific
,Cat#
65-0840-85
PE Mouse Anti-Ki-67 Set
(27)
STIM1 and STIM2 Mediate Cancer-Induced Inflammation in T Cell Acute Lymphoblastic Leukemia
Cell reports
September 11, 2018
Saint Fleur-Lominy S, Maus M, Vaeth M, Lange I, Zee I, Suh D, Liu C, Wu X, Tikhonova A, Aifantis I,
et al
.
(BM8),
Biolegend
,Cat#
123127
Anti-mouse
Ki67
(16A8),
Biolegend
,"Cat#
652404
, Cat#
652421
" Annexin V,
Biolegend
,"Cat#
640918
, Cat#
640920
" Anti-mouse CD3 (2GV6),Ventana Medical Systems,Cat# 790–4341 "Chemicals, Peptides and
(28)
Large-Scale Human Dendritic Cell Differentiation Revealing Notch-Dependent Lineage Bifurcation and Heterogeneity
Cell reports
August 14, 2018
Balan S, Arnold-Schrauf C, Abbas A, Couespel N, Savoret J, Imperatore F, Villani AC, Vu Manh TP, Bhardwaj N, Dalod M.
288-TP-025
Recombinant human GM-CSF
,
Peprotech
,Cat #
300-03
Annexin-V (Pacific blue conjugate),
Biolegend
,Cat #
640918
Propidium Iodide Solution
,
Biolegend
,Cat #
421301
Annexin V Binding Buffer
,
Biolegend
,Cat #
422201
Mouse Anti-Chicken IgA-FITC
(29)
Small extracellular vesicles and their miRNA cargo are anti-apoptotic members of the senescence-associated secretory phenotype
Aging
May 19, 2018
Terlecki-Zaniewicz L, Lämmermann I, Latreille J, Bobbili MR, Pils V, Schosserer M, Weinmüllner R, Dellago H, Skalicky S, Pum D,
et al
.
counted. For
staining
of apoptotic cells, the Pacific Blue™ Annexin-V Kit (
Biolegend
, San Diego, CA, USA,
640918
) was used. Cells and supernatants were harvested, pooled, centrifuged at 200 x g for 10 minutes (min)
(30)
Harnessing BET Inhibitor Sensitivity Reveals AMIGO2 as a Melanoma Survival Gene
Molecular cell
November 16, 2017
Fontanals-Cirera B, Hasson D, Vardabasso C, Di Micco R, Agrawal P, Chowdhury A, Gantz M, de Pablos-Aragoneses A, Morgenstern A, Wu P,
et al
.
non-adherent and adherent cells were collected and stained with AnnexinV-Pacific Blue (
BioLegend
, Cat#
640917
) and PI, following manufacturer’s protocol. For cell cycle analysis, cells were pulsed with EdU (
ThermoFisher
,
(31)
High accuracy label-free classification of single-cell kinetic states from holographic cytometry of human melanoma cells
Scientific reports
September 20, 2017
Hejna M, Jorapur A, Song JS, Judson RL.
(
ThermoFisher
87786
); iv) stained in suspension with a Pacific Blue tagged Annexin V antibody (
Biolegend
640917
) or propidium iodide (ab14083) for 20 minutes before FACS analysis; v) fixed in 4% PFA for 15 minutes
(32)
PDI is an essential redox-sensitive activator of PERK during the unfolded protein response (UPR)
Cell death & disease
August 10, 2017
Kranz P, Neumann F, Wolf A, Classen F, Pompsch M, Ocklenburg T, Baumann J, Janke K, Baumann M, Goepelt K,
et al
.
× 105 cells were incubated with 80 μg/ml PI and 9.6 μg/ml Annexin V conjugated to Pacific Blue (No.
640917
,
Biolegend
, San Diego, CA, USA) in
Annexin V binding buffer
(No.
422201
,
Biolegend
) for 15 min at room
(33)
GATA Factor-Regulated Samd14 Enhancer Confers Red Blood Cell Regeneration and Survival in Severe Anemia
Developmental cell
August 7, 2017
Hewitt KJ, Katsumura KR, Matson DR, Devadas P, Tanimura N, Hebert AS, Coon JJ, Kim JS, Dewey CN, Keles S,
et al
.
(34)
Autophagy is dispensable for Kmt2a/Mll-Mllt3/Af9 AML maintenance and anti-leukemic effect of chloroquine
Autophagy
May 4, 2017
Chen X, Clark J, Wunderlich M, Fan C, Davis A, Chen S, Guan JL, Mulloy JC, Kumar A, Zheng Y.
(35)
Biological activity of tumor-treating fields in preclinical glioma models
Cell death & disease
April 20, 2017
Silginer M, Weller M, Stupp R, Roth P.
(36)
Heterochromatin Reduction Correlates with the Increase of the KDM4B and KDM6A Demethylases and the Expression of Pericentromeric DNA during the Acquisition of a Transformed Phenotype
Journal of Cancer
January 1, 2017
Gurrion C, Uriostegui M, Zurita M.
performed according to the protocols previously described in Affymetrix cat.#65-0865 and
Biolegend
cat.#
640918
respectively. Cells were harvested and stained using Anexin pacific blue to labelled apoptotic cells.
(37)
PHD2 Is a Regulator for Glycolytic Reprogramming in Macrophages
Molecular and cellular biology
January 1, 2017
Guentsch A, Beneke A, Swain L, Farhat K, Nagarajan S, Wielockx B, Raithatha K, Dudek J, Rehling P, Zieseniss A,
et al
.
(38)
A low balance between microparticles expressing tissue factor pathway inhibitor and tissue factor is associated with thrombosis in Behçet's Syndrome
Scientific reports
December 7, 2016
Khan E, Ambrose NL, Ahnström J, Kiprianos AP, Stanford MR, Eleftheriou D, Brogan PA, Mason JC, Johns M, Laffan MA,
et al
.
(39)
Cancer-associated fibroblast-derived annexin A6+ extracellular vesicles support pancreatic cancer aggressiveness
The Journal of clinical investigation
November 1, 2016
Leca J, Martinez S, Lac S, Nigri J, Secq V, Rubis M, Bressy C, Sergé A, Lavaut MN, Dusetti N,
et al
.
(10X solution: 0.1 M HEPES pH 7.4, 1.4 M Nacl, 25 mM CaCl2) containing 5 μl of annexin V (
Biolegend
;
640918
) and 5 μl of propidium iodide (1 mg/ml, P4864; Sigma-Aldrich). Then, 400 μl of Annexin V Binding Buffer
(40)
Regional signals in the planarian body guide stem cell fate in the presence of genomic instability
Development (Cambridge, England)
May 15, 2016
Peiris TH, Ramirez D, Barghouth PG, Ofoha U, Davidian D, Weckerle F, Oviedo NJ.
100 μl binding buffer (
BioLegend
,
422201
) and stained with 5 μl Annexin V (Pacific Blue;
BioLegend
,
640918
) and 5 μl
7-AAD Viability Staining Solution
(PECy5;
BioLegend
,
420404
). Labeled cells were incubated
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Reviews
Write a Review
(1)
1/23/2019
Pacific Blue Annexin V
Anup Kumar
I am studying molecular anomalies in signaling pathways leading to cancers and the development of anti-cancer immune therapies. This antibody is good for detecting Annexin V on mouse immune cells to ...
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Pacific Blue(TM) Annexin V from BioLegend
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View Company Product Page
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