Fig 1: (A) aSyn_RT-QuIC analysis of CS-RQ-MSA and CS-RQ-PD. All samples were tested by aSyn_RT-QuIC and induced an efficient seeding activity with respect to the control (CS-RQ-CTRL). Curves represented in the graph were obtained by plotting the average fluorescence intensities of each sample against time. (B) Western blot analysis of untreated or PK digested aSyn_RT-QuIC products, collected at 30 h. Undigested samples showed the same biochemical profiles. Western blot analysis of PK digested RQ-CS-RQ-MSA1 and RQ-CS-RQ-MSA2 showed higher resistance to proteolytic digestion than RQ-CS-RQ-PD1 and RQ-CS-RQ-PD2. Membranes were immunoblotted with the AS08 358 antibody. Number on the right indicates the molecular weight. (C) Dot blot analysis of aSyn_RT-QuIC products, collected at 30 h. Dot blot analysis performed using the MJFR antibody showed the presence of a strong signal in all samples.