Anti-galectin 9/Gal-9 antibody ab69630 from Abcam

Product Specs
ItemAnti-galectin 9/Gal-9 antibody
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab69630
Antibody TypePrimary
IsotypeIgG
Conjugate/TagUnconjugated
ClonalityPolyclonal
ApplicationsImmunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
HostRabbit
Quantity100 UG
Concentration1 mg/ml
Research AreaImmuno-oncology
Gene NameLGALS9
ReactivityHuman, Mouse
Targetgalectin 9 / Gal-9

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Vps18 contributes to phagosome membrane integrity in Mycobacterium tuberculosis-infected macrophagesbioRxivOctober 10, 2023Charul Jani, Amanda Marsh, Pooja Uchil, Neha Jain, Zach R. Baskir, Owen T. Glover, David E. Root, John G. Doench, Amy K. Barczakcustom CellProfiler 4.2.1 pipelines. Antibodies: Galectin 3 (Invitrogen MA1–940), Galectin 9 (Abcam, AB69630), Vps18 (Abcam, AB178416), LAMP1 (Biolegend 328602), Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed

(2) Deficiency in Galectin-3, -8, and -9 impairs immunity to chronic Mycobacterium tuberculosis infection but not acute infection with multiple intracellular pathogensPLOS PathogensJune 23, 2023Huntly M. Morrison, Julia Craft, Rafael Rivera-Lugo, Jeffery R. Johnson, Guillaume R. Golovkine, Samantha L. Bell, Claire E. Dodd, Erik Van Dis, Wandy L. Beatty, Shally R. Margolis, et al.1:1000, secondary 1:5000), anti-Gal-8 (Abcam ab69631, primary 1:500, secondary 1:5000), anti-Gal-9 (Abcam ab69630, primary 1:500, secondary 1:5000), anti-β-actin (SCBT sc-47778, primary 1:1000, secondary 1:5000), anti-FLAG

(3) Cancer cell intrinsic TIM-3 induces glioblastoma progressioniScienceNovember 18, 2022Guo Q, Shen S, Guan G, Zhu C, Zou C, Cao J, Cheng W, Xu X, Yu J, Lin Z, et al.AB_2127460 IL6,R & D Systems,AF206; RRID: AB_354392 Flag,Sigma-Aldrich,F1804; RRID: AB_262044 Galectin-9,Abcam,ab69630; RRID: AB_1268942 TIM-3,Proteintech,60355; RRID: AB_2881464 TIM-3,Abcam,ab47997; RRID: AB_883007 TIM-3

(4) Single-cell characterization of malignant phenotypes and microenvironment alteration in retinoblastomaCell Death & DiseaseMay 6, 2022Cheng Wu, Jiaqi Yang, Wei Xiao, Zehang Jiang, Shuxia Chen, Dianlei Guo, Ping Zhang, Chunqiao Liu, Huasheng Yang, Zhi XieTAMs, CD86(14-0862; Invitrogen), CD163(ab182422; Abcam), PTPRC (CD45) (14-0451; Invitrogen) and LGALS9 (ab69630; Abcam). Alexa Fluor secondary antibodies (Invitrogen) were used for detection of primary antibodies.

(5) Obesity-induced galectin-9 is a therapeutic target in B-cell acute lymphoblastic leukemiaNature communicationsMarch 3, 2022Lee M, Hamilton JAG, Talekar GR, Ross AJ, Michael L, Rupji M, Dwivedi B, Raikar SS, Boss J, Scharer CD, et al.B-ALL cell lysates were prepared following the same protocol77 and lysates were probed for GAL9 (cat# ab69630, 1:500, Abcam), TIM3(cat# ab47997, 1:1000, Abcam), and β-actin (cat# 4967, 1:5000, cell signaling).

(6) HIV-1 and methamphetamine alter galectins -1, -3, and -9 in human monocyte-derived macrophagesJournal of NeuroVirologyFebruary 17, 2022Kinga Grabowska, Katarzyna Macur, Sarah Zieschang, Lubaba Zaman, Nicole Haverland, Andrew Schissel, Brenda Morsey, Howard S. Fox, Pawel Ciborowskianti-galectin-3 (B2C10) (Santa Cruz Biotechnology, Dallas, TX, USA) Abs (1:500) or rabbit anti-galectin-9 (ab69630) (Abcam) Abs (1:200) for 1.5 h. Next, cells were incubated with the suitable secondary antibodies Alexa

(7) Serum Levels of Galectin-9 are Increased in Cervical Cancer Patients and are Higher in Advanced Clinical StagesOncoTargets and therapyJanuary 1, 2022Reyes-Vallejo T, Conde-Rodríguez I, Serna-Villalobos J, Ramírez-Díaz I, Pérez-Villalobos G, Delgado-López G, Vazquez-Zamora VJ, Gutiérrez-Quiroz CT, Ávila-Jiménez L, García-Carrancá A, et al.binding, slides were incubated for 1 h in 1% BSA in PBS. Sections were incubated with galectin-9 antibody (ab69630, Abcam, Cambridge, MA USA) diluted at 1:400 overnight at 4°C. Then, the sections were incubated with

(8) Low Distribution of TIM-3+ Cytotoxic Tumor-Infiltrating Lymphocytes Predicts Poor Outcomes in Gastrointestinal Stromal TumorsJournal of Immunology ResearchFebruary 17, 2021Chun Zhuang, Bo Ni, Zi-Zhen Zhang, Wen-Yi Zhao, Lin Tu, Xin-Li Ma, Lin-Xi Yang, Hui Cao, Ming Wang(CST, #85336, 1 : 200), CD56 (CST, #3576, 1 : 200), TIM-3 (Abcam, ab185703, 1 : 200), and Gal-9 (Abcam, ab69630, 1 : 200). 2.2. Tumor Specimens Archived formalin-fixed paraffin-embedded tumor tissue from 299 GIST

(9) BDNF expression in GISTs predicts poor prognosis when associated with PD-L1 positive tumor-infiltrating lymphocytesOncoimmunologyJanuary 1, 2021Tian GA, Xu WT, Sun Y, Wang J, Ke Q, Yuan MJ, Wang JJ, Zhuang C, Gong Q.(CST, # 13684, 1: 200), PD-1 (CST, 43248, 1: 100), TIM3 (Abcam, ab185703, 1: 200), Galectin 9 (Abcam, ab69630, 1: 200), CD3 (Servicebio, GB13440, 1: 100), and CD8 (CST, 85336, 1: 100). Figure 1.IHC scores for BDNF

(10) Splenic CD4⁺ and CD8⁺ T-cells highly expressed PD-1 and Tim-3 in cirrhotic patients with HCV infection and portal hypertensionInternational journal of immunopathology and pharmacologyJanuary 1, 2021Huang N, Zhou R, Chen H, Zhang S, Li J, Wei W, Sun J, Ren S, Li B, Deng H, et al.molecules PD-1 (EPR4877(2), 1:250), PD-L1 (EPR1161(2), 1:100), Tim-3 (ab185703, 1:100), Galectin-9 (ab69630, 4 μg/mL), CD28 (ab243228, 1:500), CD80 (ab134120, 1:200) (all from Abcam, Cambridge, MA, USA), PD-L2

(11) SUV39H1-DNMT3A-mediated epigenetic regulation of Tim-3 and galectin-9 in the cervical cancerCancer Cell InternationalDecember 1, 2020Li Zhang, Sijuan Tian, Minyi Zhao, Ting Yang, Shimin Quan, Qing Yang, Lihua Song, Xiaofeng Yang,Cell Signaling Technology,1:1000,8729,WB Tim-3,Abcam,1:50,ab47997,IF Tim-3,Abcam,1:250/1:250,ab185703,"WB, IHC" Galectin-9,Abcam,1:200,ab123712,WB Galectin-9,Abcam,1:250/1:200,ab69630,"IF, IHC" DNMT3A,Abcam,1:50/1:250,ab13537,"ChIP, WB" IgG,Cell Signaling Technology,1:500,2729,ChIP Histone

(12) Galectin-3 not Galectin-9 as a candidate prognosis marker for hepatocellular carcinomaPeerJSeptember 16, 2020Fei Kong, Meishan Jin, Donghui Cao, Zhifang Jia, Yawen Liu, Jing Jiang3% H2O2, all sections were incubated with anti-human galectin-9 polyclonal antibody (1:250 diluted, ab69630, Abcam, UK) and galectin-3 monoclonal antibody (1:100 diluted, ZA-0534, Zhongshan jinqiao, China) for

(13) Enzyme Replacement Therapy Can Reverse Pathogenic Cascade in Pompe DiseaseMolecular therapy. Methods & clinical developmentSeptember 11, 2020Meena NK, Ralston E, Raben N, Puertollano R.purchased from BD Transduction Laboratories. SQSTM1/p62 (#ab56416; mouse monoclonal), galectin 9 (#ab69630; rabbit polyclonal), Ubiquitin K48 (Linkage Specific) (#ab140601; rabbit monoclonal), and glyceraldehyde-3-phosphate

(14) Immune checkpoints bone marrow expression as the predictor of clinical outcome in myelodysplastic syndromeLeukemia Research ReportsJune 28, 2020Nikolai Tcvetkov et al.the following antigens: PD-1 (ab52587), PD-L1 (ab205921), PD-L2 (ab200377), LAG-3 (ab40465), Gal-9 (ab69630), TIM-3 (ab185703), CD80 (ab64116). We used monoclonal antibodies produced by Abcam (1 Kendall Square,

(15) The Association Between Immune Characteristic and Clinical Pathology in Chinese Patients with Adenocarcinoma of Esophagogastric JunctionCancer Management and ResearchMay 11, 2020Yangyang Wang, Shuchang Wang, Chunchao Zhu, Hui Cao, Zizhen Zhang, Enhao ZhaoUK, ab205921), PD-1 (1: 100, CST,USA, 43248), Tim-3 (1:200, CST,USA, 45208), Gal-9 (1:250, Abcam, UK, ab69630), CD3 (1: 200, Wuhan Goodbio Technology Co., Ltd., China), CD8 (1: 100, Wuhan Goodbio Technology Co., Ltd.,

(16) AMPK, a key regulator of metabolism and autophagy, is activated by lysosomal damage via a novel galectin-directed ubiquitin signal transduction systemMolecular CellMarch 1, 2020Jingyue Jia et al.(1:200 for IF), LAMP1 (#9091) (1:500 for IF), AXIN (#2087). Antibodies from Abcam were Galectin 9 (ab69630; 1:500 WB; 1:100 IF), USP9x (ab19879; 1:1000 WB), GFP (ab290; 1:1000 WB), GFP (ab38689; 2 μg/mL for

(17) Plasma Transfusion Promoted Reprogramming CD4⁺ T Lymphocytes Immune Response in Severe Sepsis Mice Model Through Modulating the Exosome Protein Galectin 9Cell transplantationJanuary 1, 2020Zhang L, Zhang JP, Liu Y, Wang H, Cheng Y, Wang JH, Zhang WJ, Li ZZ, Guo JR.4478359; Invitrogen, USA), ExoAb Antibody Kit (catalog: EXOAB-KIT-1; SBI, USA), antimouse Galectin 9 (catalog: ab69630; Abcam, Wuhan, China), transmission electron microscope (catalog: HT7700; HITACHI, Japan), mouse lymphocyte

(18) Association between Tim‑3 and Gal‑9 expression and gastric cancer prognosisOncology reportsOctober 1, 2018Wang Y, Zhao E, Zhang Z, Zhao G, Cao H.cat. no. 45208; Cell Signaling Technology, Inc., Danvers, MA, USA), Gal‑9 (dilution, 1:250; cat. no. ab69630; Abcam, Cambridge, UK), CD3 (dilution, 1:200; cat. no. GB11014; Wuhan Goodbio Technology Co., Ltd.,

(19) Galectins Control mTOR in Response to Endomembrane DamageMolecular CellApril 5, 2018Jingyue Jia et al.from Sigma Aldrich; GFP (ab290) (1:1000 for WB), beta-Tubulin (ab6046) (1:1000 for WB), Galectin-9 (ab69630) (1:1000 for WB) from Abcam, LKB1 (ab61122) (1:1000 for WB), CAMKK2 (ab168818) (1:1000 for WB); Galectin-8

(20) T-cell immunoglobulin and mucin domain-containing protein-3 and galectin-9 protein expression: Potential prognostic significance in esophageal squamous cell carcinoma for Chinese patientsOncology lettersDecember 1, 2017Hou N, Ma J, Li W, Zhao L, Gao Q, Mai L.3 min. Following incubation with the primary human galectin-9 polyclonal antibody (dilution, 1:250; ab69630; Abcam, Cambridge UK) and Tim-3-phycoerythrin (dilution, 1:200; 565560; BD Biosciences, Franklin Lakes,

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Anti-galectin 9/Gal-9 antibody from Abcam

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Anti-galectin 9/Gal-9 antibody from Abcam

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