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Antibodies
Antibodies
Anti-CERT antibody
Anti-CERT antibody from Abcam
Be the first to write a review!
Citations:
(13)
Supplier Page
Supplier Page from
Abcam for
Anti-CERT antibody
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-CERT antibody
Company
Abcam
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
ab72536
Antibody Type
Primary
Applications
Immunoprecipitation, Western blot
Conjugate/Tag
Unconjugated
Host
Rabbit
Quantity
100 UL
Isotype
IgG
Clonality
Polyclonal
Concentration
1 mg/ml
Gene Name
COL4A3BP
Reactivity
Human
Target
CERT
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (13)
(1)
Herpes simplex virus 1 protein pUL21 alters ceramide metabolism by activating the interorganelle transport protein CERT
The Journal of biological chemistry
November 1, 2022
Benedyk TH, Connor V, Caroe ER, Shamin M, Svergun DI, Deane JE, Jeffries CM, Crump CM, Graham SC.
listed dilutions were used
for immunoblotting
: rabbit
anti-CERT
(
1:10,000
dilution;
Abcam
; catalog no.:
ab72536
),
mouse
anti-pUL21 (
1:50
dilution;
clone
no.: 1F10-D12) (18) for Figure 1F (note: this monoclonal antibody
(2)
A genome-wide CRISPR-Cas9 screen identifies CENPJ as a host regulator of altered microtubule organization during Plasmodium liver infection
Cell chemical biology
September 15, 2022
Vijayan K, Arang N, Wei L, Morrison R, Geiger R, Parks KR, Lewis AJ, Mast FD, Douglass AN, Kain HS,
et al
.
RRID:AB_2799298" Anti-
CISD1
,
Cell Signaling Technology
,"Cat#
83775
, RRID:AB_2800031" Anti-COL4A3BP,
Abcam
,"Cat#
ab72536
, RRID:AB_2082802"
Anti-FMN2
,
Abcam
,"Cat#
ab72052
, RRID:AB_1209289" Anti-
IREB2
,
Cell Signaling Technology
,"Cat#
(3)
Involvement of a Cluster of Basic Amino Acids in Phosphorylation-Dependent Functional Repression of the Ceramide Transport Protein CERT
International Journal of Molecular Sciences
August 2, 2022
Asako Goto, Daichi Egawa, Nario Tomishige, Toshiyuki Yamaji, Kentaro Shimasaki, Keigo Kumagai, Kentaro Hanada
Diagnostics; anti-His (#D291-3S) from Medical & Biological Laboratories Co., Ltd.;
anti-CERT
from
Abcam
(#
ab72536
);
anti-GM130
(#
610823
) from
BD Biosciences
; and anti-GAPDH (#016-25523) from Fujifilm Wako Pure Chemical
(4)
Compartmentalization of casein kinase 1 γ CSNK1G controls the intracellular trafficking of ceramide
iScience
July 15, 2022
Goto A, Sakai S, Mizuike A, Yamaji T, Hanada K.
details Antibodies and reagents Anti-HA antibody (#3F10) was purchased from Roche Diagnostics;
anti-CERT
(#
ab72536
) from
Abcam
;
chicken
antibody against VAP (Kumagai et al., 2014) anti-TGOLN2/TGN46 (#
A304-434A
) from
(5)
Hyperosmotic Stress Induces Phosphorylation of CERT and Enhances Its Tethering throughout the Endoplasmic Reticulum
International Journal of Molecular Sciences
April 5, 2022
Kentaro Shimasaki, Keigo Kumagai, Shota Sakai, Toshiyuki Yamaji, Kentaro Hanada
standards. After blocking, the
membranes
were immunoblotted using rabbit polyclonal
anti-CERT
antibody (
ab72536
,
Abcam
, Cambridge, UK), monoclonal anti-HA antibody (
clone
3F10) conjugated with horseradish peroxidase
(6)
Intellectual-disability-associated mutations in the ceramide transport protein gene CERT1 lead to aberrant function and subcellular distribution
Journal of Biological Chemistry
November 1, 2021
Norito Tamura, Shota Sakai, Loreto Martorell, Roser Colomé, Aya Mizuike, Asako Goto, Juan Darío Ortigoza-Escobar, Kentaro Hanada
for establishment of the KI cell line.
For western blotting
analysis, rabbit polyclonal
anti-CERT
(
ab72536
;
1:500
,
Abcam
),
rat
monoclonal anti-HA conjugated with horseradish peroxidase (HRP;
clone
3F10;
1:5000
,
(7)
pUL21 is a viral phosphatase adaptor that promotes herpes simplex virus replication and spread
PLoS pathogens
August 1, 2021
Benedyk TH, Muenzner J, Connor V, Han Y, Brown K, Wijesinghe KJ, Zhuang Y, Colaco S, Stoll GA, Tutt OS,
et al
.
following antibodies with listed dilutions were used
for immunoblotting
:
anti-CERT
1:10,000
(
Abcam
,
ab72536
), anti-pUL21
1:50
(this study, see below), anti-PP1α
1:1000
(
Santa Cruz
,
sc-271762
), anti-PP1β
1:1000
(8)
Intellectual disability-associated gain-of-function mutations in CERT1 that encodes the ceramide transport protein CERT
PLOS ONE
December 21, 2020
Hiroaki Murakami, Norito Tamura, Yumi Enomoto, Kentaro Shimasaki, Kenji Kurosawa, Kentaro Hanada
For Western blotting
analysis, the following primary antibodies were used: rabbit polyclonal
anti-CERT
(
ab72536
;
Abcam
, Cambridge, MA, USA), anti-HA conjugated with horseradish peroxidase (HRP;
clone
3F10; Roche,
(9)
Nanoscale imaging of bacterial infections by sphingolipid expansion microscopy
Nature Communications
December 2, 2020
Ralph Götz, Tobias C. Kunz, Julian Fink, Franziska Solger, Jan Schlegel, Jürgen Seibel, Vera Kozjak-Pavlovic, Thomas Rudel, Markus Sauer
rb (US biological, dilution
1:200
), anti-Prx3 (
Origene
,
TA322470
, dilution
1:100
),
anti-CERT
(
Abcam
,
ab72536
,
1:100
) and anti-LPS (
BioRAD
,
MCA2718
, dilution
1:200
). After that, the cells were washed 3x in 1× PBS
(10)
Intracellular localization of diacylglycerols and sphingolipids influences insulin sensitivity and mitochondrial function in human skeletal muscle
JCI insight
February 8, 2018
Perreault L, Newsom SA, Strauss A, Kerege A, Kahn DE, Harrison KA, Snell-Bergeon JK, Nemkov T, D'Alessandro A, Jackman MR,
et al
.
ATGL
, cs
2138
; Lipin 1, cs5195),
Santa Cruz
(
FIS1
,
sc376447
; PKCt,
sc1875
; PKC BII,
sc210
),
Abcam
(CERT,
ab72536
; SMPD4,
ab107760
),
Abgent
Inc. (DKGδ,
AP8126b
), R&D Systems Inc. (
SMPD3
,
AF7184
), and
Novus Biologicals
(11)
Role of Intracellular Lipid Logistics in the Preferential Usage of Very Long Chain-Ceramides in Glucosylceramide
International Journal of Molecular Sciences
October 21, 2016
Toshiyuki Yamaji, Aya Horie, Yuriko Tachida, Chisato Sakuma, Yusuke Suzuki, Yasunori Kushi, Kentaro Hanada
antibody was from Bethyl Laboratories (Montgomery, TX, USA). Rabbit anti-COL4A3BP (
CERT) antibody
(
ab72536
) was from
Abcam
(Cambridge, UK).
Mouse
anti-UGCG IgG (1E5) was from Abnova (Taipei, Taiwan). Blasticidin
(12)
Growth of the Mammalian Golgi Apparatus during Interphase
Molecular and Cellular Biology
September 15, 2016
Alex T.-W. Sin, Rene E. Harrison
10% FBS and 0.3 M glycine in 0.1% PBS–Tween 20. Anti-COL4A3BP antibody (
1:200
) (
Abcam
, catalog no.
ab72536
) was then applied for 24 h. All secondary
staining
were carried out with Cy2-, Cy3-, or Cy5-conjugated antibodies
(13)
Natural products reveal cancer cell dependence on oxysterol-binding proteins
Nature Chemical Biology
September 1, 2011
Anthony W G Burgett et al.
c-Myc–specific (9E10)
mouse
antibodies (Santa Cruz Biotechnologies); polyclonal COL4A3BP/CERT–specific (
ab72536
) rabbit antibodies (
Abcam
); monoclonal p230-specific
mouse
antibodies (BD Biosciences). Cell lines HCT-116
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Reviews
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Anti-CERT antibody from Abcam
Supplier Page
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