SSEA4 (MC813) Mouse mAb 4755S from Cell Signaling Technology

Product Specs
ItemSSEA4 (MC813) Mouse mAb
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number4755S
Quantity100 µl
ApplicationsIF-IC, FC-FP, FC-L
ReactivityH
HostMouse
ClonalityMonoclonal
Research AreaDevelopmental Biology
TargetSSEA4
IsotypeIgG3
CloneMC813

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Using Ribonucleoprotein-based CRISPR/Cas9 to Edit Single Nucleotide on Human Induced Pluripotent Stem Cells to Model Type 3 Long QT Syndrome (SCN5A±)Stem Cell Reviews and ReportsNovember 1, 2023Ning Ge, Min Liu, Rui Li, Nicholas M. Allen, Joseph Galvin, Sanbing Shen, Timothy O’Brien, Terence W. Prendiville(Cell Signaling Technology, #3580)Mouse anti-SSEA4 (IgG3) primary antibody (Cell Signaling Technology, #4755)Mouse anti-TRA-1–60 (IgM) primary antibody (Cell Signaling Technology, #4746)Mouse anti-TRA-1–81 (IgM)

(2) The pseudoenzyme ADPRHL1 affects cardiac function by regulating the ROCK pathwayStem Cell Research & TherapyOctober 26, 2023Lei Tian, Tianwei Guo, Fujian Wu, Rui Bai, Sinan Ai, Hongyue Wang, Yuanxiu Song, Min Zhu, Youxu Jiang, Shuhong Ma, et al.with the following primary antibody: anti-SOX2 antibody (CST, #2748, USA), anti-SSEA4 antibody (CST, #4755, USA), anti-CX43 antibody (CST, #3512, USA), anti-Paxillin antibody (CST, #2542, USA) overnight at 4

(3) Development of MAPT S305 mutation models exhibiting elevated 4R tau expression, resulting in altered neuronal and astrocytic functionbioRxivJune 9, 2023K R Bowles, D A Pugh, C Pedicone, L Oja, S A Weitzman, Y Liu, J L Chen, M D Disney, A M Goatewere used: TRA-1–81 (Cell Signaling Technologies 4745, 1:1000), SSEA4 (Cell Signaling Technologies 4755, 1:1000), TRA-1–60 (Cell Signaling Technologies 4746, 1:1000), Nanog (Cell Signaling Technologies D73G4,

(4) A Mutant Methionyl-tRNA Synthetase-Based toolkit to assess induced-Mesenchymal Stromal Cell secretome in mixed-culture disease modelsResearch squareMay 3, 2023Burgess JD, Amerna D, Norton ES, Parsons TM, Perkerson RB 3rd, Faroqi AH, Wszolek ZK, Cazares HG, Kanekiyo T, Delenclos M, et al.(goat),"R&D Systems (967330), from StemXVivo Endoderm Kit (SCO19B)",1μg/100μL (ICC) SSEA4,Cell Signaling (#4755),1:500 (ICC) Oct-4,Cell Signaling (#2750),1:200 (ICC) "CD73 Monoclonal Antibody (AD2), APC",eBioscience

(5) Induced pluripotent stem cell-derived extracellular vesicles promote wound repair in a diabetic mouse model via an anti-inflammatory immunomodulatory mechanismbioRxiv : the preprint server for biologyMarch 23, 2023Levy D, Abadchi SN, Shababi N, Ravari MR, Pirolli NH, Bergeron C, Obiorah A, Mokhtari-Esbuie F, Gheshlaghi S, Abraham JM, et al.Oct-4 (2890S, C52G3; Cell Signaling Technology Incorporated, Danvers, MA, USA) at a 1:500, or SSEA-4 (4755S, MC813; Cell Signaling Technology Incorporated, Danvers, MA, USA) at a 1:200 dilution and incubation

(6) Fabrication Scaffold with High Dimensional Control for Spheroids with Undifferentiated iPS Cell PropertiesCellsJanuary 11, 2023Togo H, Terada K, Ujitsugu A, Hirose Y, Takeuchi H, Kusunoki M.MA, USA), mouse monoclonal anti-TRA-1-81 (1:1000; 4745, CST), mouse monoclonal anti-SSEA4 (1:1000; 4755, CST), rabbit anti-NANOG (1:500; 4903, CST), rabbit monoclonal anti-FOXA2/HNF3β (1:400; 8186S, CST),

(7) Stabilization of hESCs in two distinct substates along the continuum of pluripotencyiScienceDecember 22, 2022Chen Dekel, Robert Morey, Jacob Hanna, Louise C. Laurent, Dalit Ben-Yosef, Hadar Amirused at the indicated dilutions: mouse anti-OCT4 (sc-5279, Santa-Cruz, 1:60), mouse anti-SSEA4 (CST-4755S, cell signaling technology, 1:200), mouse anti-TRA-1-60 (ab16288, Abcam, 1:200) and rabbit anti-TFE3

(8) Transcriptomic Analysis of Human Fragile X Syndrome Neurons Reveals Neurite Outgrowth Modulation by the TGFβ/BMP PathwayInternational Journal of Molecular SciencesAugust 17, 2022Liron Kuznitsov-Yanovsky, Guy Shapira, Lital Gildin, Noam Shomron, Dalit Ben-YosefTriton X 100 in PBS for permeabilization. Cells were then incubated with primary antibodies (anti-SSEA4, CST-4755, Cell Signaling Technology, Danvers, MA, USA; anti-TRA-1-60, ab16288, Abcam, Cambridge, UK; anti-OCT4,

(9) iPS-derived neural stem cells for disease modeling and evaluation of therapeutics for mucopolysaccharidosis type IIExperimental Cell ResearchMarch 1, 2022Junjie Hong, Yu-Shan Cheng, Shu Yang, Manju Swaroop, Miao Xu, Jeanette Beers, Jizhong Zou, Wenwei Huang, Juan J. Marugan, Xiujun Cai, et al.from the Developmental Studies Hybridoma Bank (Iowa City, IW). Antibodies for Sox2 (#4900S) SSEA4 (#4755S), Nanog (#4903), and Tra-1–60 (#4746) were purchased from Cell Signaling Technology (Danvers, MA). Paraformaldehyde

(10) Unraveling three-dimensional chromatin structural dynamics during spermatogonial differentiationJournal of Biological ChemistryFebruary 1, 2022Yi Zheng, Lingkai Zhang, Long Jin, Pengfei Zhang, Fuyuan Li, Ming Guo, Qiang Gao, Yao Zeng, Mingzhou Li, Wenxian Zeng1% fetal bovine serum and 2 mM EDTA) and then incubated with the mouse anti-SSEA4 antibody (1: 50; 4755S, Cell Signaling Technology) on ice for 30 min, followed by washing and incubation with the Alexa fluor

(11) p53 Promotes Differentiation of Cardiomyocytes from hiPSC through Wnt Signaling-Mediated Mesendodermal DifferentiationInternational journal of stem cellsNovember 30, 2021Liu Y, Zhang P, Huang W, Liu F, Long D, Peng W, Dang X, Zeng X, Zhou R.Cell Signaling Technology, USA), anti-NANOG antibody (#3580, Cell Signaling Technology, USA), anti-SSEA4 antibody (#4755, Cell Signaling Technology, USA), anti-TRA-1-60 antibody (#4746, Cell Signaling Technology, USA), anti-MKI67

(12) CDKN2A-Mutated Pancreatic Ductal Organoids from Induced Pluripotent Stem Cells to Model a Cancer Predisposition SyndromeCancersOctober 13, 2021Merkle J, Breunig M, Schmid M, Allgöwer C, Krüger J, Melzer MK, Bens S, Siebert R, Perkhofer L, Azoitei N, et al.(Santa Cruz Biotechnology, Dallas, TX, USA, sc-5279, 1:200), NANOG (Cell Signaling, 3580, 1:500), SSEA4 (Cell Signaling, 4755, 1:500), PDX1 (R&D, AF2419, 1:500), NKX6-1 (DSHB, F55A12, 1:150), and as secondary antibodies Alexa-Fluor

(13) Generation of two gene corrected human isogenic iPSC lines (NCATS-CL6104 and NCATS-CL6105) from a patient line (NCATS-CL6103) carrying a homozygous p.R401X mutation in the NGLY1 gene using CRISPR/Cas9Stem Cell ResearchOctober 1, 2021Ivan Pavlinov et al."Cell signaling, Cat# 4903, RRID: AB_10559205" Pluripotency Markers,Mouse anti-SSEA4,1:500,"Cell signaling, Cat# 4755, RRID: AB_1264259" Secondary Antibodies,Donkey anti-Mouse IgG (Alexa Fluor 488),1:400,"Thermo Fisher,

(14) Modeling of dilated cardiomyopathy by establishment of isogenic human iPSC lines carrying phospholamban C25T (R9C) mutation (UPITTi002-A-1) using CRISPR/Cas9 editingStem Cell ResearchOctober 1, 2021Robert J. Barndt, Ning Ma, Ying Tang, Michael P. Haugh, Laila S. Alamri, Stephen Y. Chan, Haodi WuCat#4903S RRID: AB_10559205 Pluripotency marker,SSEA (MC813) Mouse mAb,1:500,Cell Signaling Technology Cat#4755S RRID: AB_1264259 Pluripotency marker,TRA-1–60(S) Mouse mAb,1:400,Cell Signaling Technology Cat#4746S

(15) Generation of an induced pluripotent stem cell line (TRNDi012-B) from Fibrodysplasia Ossificans Progressiva (FOP) patient carrying a heterozygous mutation c. 617G > A in the ACVR1 geneStem cell researchJuly 1, 2021Huang X, Roeder A, Li R, Beers J, Liu C, Zou J, Yu PB, Zheng W.Cat# A13998, RRID: AB_2534182" Pluripotency Markers,Mouse anti-SSEA4,1:1000,"Cell signaling, Cat# 4755, RRID: AB_1264259" Secondary Antibodies,Donkey anti-Mouse IgG (Alexa Fluor 488),1:400,"Thermo Fisher,

(16) An induced pluripotent stem cell line (NCATS-CL9075) from a patient carrying compound heterozygote mutations, p.R390P and p.L318P, in the NGLY1 geneStem cell researchJuly 1, 2021Pradhan M, Farkhondeh A, Cheng YS, Xu M, Beers J, Zou J, Liu C, Might M, Rodems S, Baumgärtel K, et al.A13998, RRID: AB_2534182" Pluripotency Primary Marker,Mouse anti-SSEA4,1:1000,"Cell signaling, Cat# 4755, RRID: AB_1264259" Secondary Antibodies,Donkey anti-Mouse IgG (Alexa Fluor 488),1:400,"Thermo Fischer,

(17) Generation of an induced pluripotent stem cell line (TRNDi031-A) from a patient with Alagille syndrome type 1 carrying a heterozygous p. C312X (c. 936 T > A) mutation in JAGGED-1Stem cell researchJuly 1, 2021Brooks BM, Pradhan M, Cheng YS, Gorshkov K, Farkhondeh A, Chen CZ, Beers J, Liu C, Baumgaertel K, Rodems S, et al.# A13998",AB_2534182 Pluripotency Markers,Mouse anti-SSEA4,1:500,"Cell Signaling Technology, Cat # 4755",AB_1264259 Pluripotency Markers,Mouse anti-TRA-1–60,1:500,"Cell Signaling Technology, Cat # 4746",AB_1264259 Secondary

(18) Modeling plasticity and dysplasia of pancreatic ductal organoids derived from human pluripotent stem cellsCell stem cellJune 3, 2021Breunig M, Merkle J, Wagner M, Melzer MK, Barth TFE, Engleitner T, Krumm J, Wiedenmann S, Cohrs CM, Perkhofer L, et al.(Cell Signaling Cat#3580, 1:500), SSEA4 (stained without permeabilization step, Cell Signaling Cat#4755, 1:500) and Alexa-conjugated secondary antibodies. Cells on IBIDI-plates were imaged on a Keyence Biozero

(19) Generation of Alagille syndrome derived induced pluripotent stem cell line carrying heterozygous mutation in the JAGGED-1 gene at splicing site (Chr20: 10,629,709C>A) before exon 11Stem cell researchMay 1, 2021Zhu W, Cheng YS, Xu M, Farkhondeh A, Beers J, Zou J, Liu C, Baumgaertel K, Rodems S, Zheng W."Cell signaling, Cat# 4903, RRID: AB_10559205" Pluripotency Markers,Mouse anti-SSEA4,1:500,"Cell signaling, Cat# 4755, RRID: AB_1264259" Secondary Antibodies,Donkey anti-Mouse IgG (Alexa Fluor 488),1:400,"Thermo Fischer,

(20) Generation of an induced pluripotent stem cell line (TRNDi030-A) from a patient with Farber disease carrying a homozygous p. Y36C (c. 107 A>G) mutation in ASAH1Stem cell researchMay 1, 2021Brooks BM, Yeh CD, Beers J, Liu C, Cheng YS, Gorshkov K, Zou J, Zheng W, Chen CZ.A13998",RRID: AB_2534182 Pluripotency Markers,Mouse anti-SSEA4,1:1000,"Cell Signaling Technology, Cat # 4755",RRID: AB_1264259 Secondary Antibodies,Donkey anti-Mouse IgG (Alexa Fluor 488),1:400,"Thermo Fisher Scientific

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SSEA4 (MC813) Mouse mAb

SSEA4 (MC813) Mouse mAb from Cell Signaling Technology

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Cell Signaling Technology

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SSEA4 (MC813) Mouse mAb from Cell Signaling Technology

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