Fig 1: Transcriptomic Profiling of S3- and S5-Stratifying Gbx2+ AC Subtypes Identifies Molecular Differences(A) Differentially expressed genes (DEGs) identified by RNA-seq between S3- and S5-Gbx2+ ACs are displayed in a volcano plot (negative fold change, S3 enriched; positive fold change, S5 enriched). The blue points represent genes that have significant differences in between the S3- and S5- Gbx2+ AC subtypes (p < 0.05, false discovery rate [FDR], gray line).(B) Fold change in expression determined by RNA-seq for known S3-specific markers in S3 (blue bars) and S5 (white bars) Gbx2+ AC subtypes. Each point (black diamond) represents a single sample/animal.(C) The mean expression of neurotransmitter markers from RNA-seq between S3- and S5-Gbx2+ ACs displayed in a heatmap. Neurotransmitter markers are subdivided into GABA synthesis (Gad1, Gad2, Abat, Aldh1a1), GABA transport (Slc32a1, Slc6a1), glycine transport (Slc6a9, Slc6a5), glutamate transport (Slc17a7, Slc17a6, Slc17a8, Slc17a5), cholinergic synthesis and transport (Chat, Slc5a7), catecholamine synthesis (Th, Dbh, Pnmt), serotonin synthesis (Tph1, Tph2), histamine synthesis (Hdc), monoamine transport (Slc18a1, Slc18a2), and neuropeptides (Tac1, Crh).(D and E) Heatmaps comparing the mean expression of highly and differentially expressed (D) transcription factors, (E) cell adhesion and axon guidance receptors, and (F) neurotransmitter receptors and ion channels. Cpm, counts per million reads.The data are represented as means.See also Figure S4.