Fig 1: Heterogeneity of epithelial cells among the three TET tumor types.(a) UMAP visualization of total cells from samples in each group, colored by the cell types of epithelial cells and other cells (n = 2, 2 and 2 for T1, T2 and T3, respectively). (b) Bar plot showing the frequencies of epithelial cells among the TET groups (n = 2, 2 and 2 for T1, T2 and T3, respectively). (c) Violin plots showing the expression of the marker genes for CSCs on epithelial cells among different types of TETs. (d) Pseudotime trajectory for epithelial cells from all samples in a two-dimensional state-space defined by Monocle2, colored by group. (e) UMAP plot of all epithelial cells from the normal human thymus (n = 1) and TET samples (n = 6), colored by the identified cell subpopulation (left). Same UMAP plot, colored by group (right). (f) Dot plot of the expression of marker genes in epithelial cell types. (g) UMAP visualization of the expression of marker genes used for epithelial cell cluster identification. The color scale represents normalized expression. Gray to brown: low to high expression. (h) Composition of the epithelial cell compartment showing average frequencies of major epithelial cell subpopulations for each group of TETs; each color represents an epithelial cell subset (n = 2, 2 and 2 for T1, T2 and T3, respectively). (i) Representative IF staining images showing CCL25 (pink), CHI3L1 (red), KRT14 (green) and DAPI (nuclei, blue) in TET samples (scale bar: 100 µm). Experiment was performed in three independent samples for each group with similar results. Source data are provided as a Source Data file.