Fig 2: GSK3-inhibited NK cells show high activity in AML mouse model systems.(a,b) NSG mice were injected with OCI-AML3 cells followed by weekly injections of vehicle, expanded NK cells, or expanded NK cells that were pre-treated with SB for 16 h. The per cent human AML cells (CD56- CD45+) were detected by flow cytometry from (a) spleen and (b) bone marrow after 6 weeks (c) NSG mice were injected with primary AML cells followed by weekly injections of vehicle, expanded NK cells, or expanded NK cells that were pre-treated with compound 117 for 16 h. The per cent human AML cells (CD56- CD45+) were detected by flow cytometry from the bone marrow after 5 weeks. (unpaired Student's t-test ***P<0.001, **P<0.01). (d) NSG mice were injected with primary human AML cells followed by four (bi-weekly) injections of vehicle, expanded NK cells or expanded NK cells pre-treated with compound 117 for 16 h. Kaplan–Meier survival curves of mice are shown. ?2 log-rank test was performed for statistical significance. **P<0.01 for AML+NK versus AML+ (NK+117) groups. Experiments is performed two times. In all mice experiments, five mice per group were used.

Fig 3: A unique ILC population exists in human tonsils.a Representative conventional flow cytometry plots from an adult tonsil showing the hierarchical phenotype gating strategy from singlet lymphocytes to the ILC1 (green), ILC2 (pink), and ILC3 (blue) and a unique ILC subset (ILCFR) (red) populations indicated by arrows and color-coded gates. (Lin = CD4, CD11b, CD11c, CD14, CD16). b Graph shows frequency of ILC1, ILC2, ILC3, and ILCFR among CD45+ human tonsil mononuclear cells (n = 7 or 8 biologically independent tonsils per group, 3 independent experiments) (Ordinary one-way ANOVA with multiple comparisons; F = 5.2; **p = 0.0058; Mean ± SD). c Analysis of surface and transcription markers on ILCFR by flow cytometry. Gray histograms depict fluorescence minus one (FMO) control of each antibody. Red lines indicate ILCFR. Blue lines denote ILC1 (for CD56, Tbet), ILC2 (KLRG1, GATA3), ILC3 (NKp44, ID3, CD127, ILR12RB1, CD7, CCR6, IL-2R?), and T cells (CD4) (n = 10 biologically independent tonsils per group, 3 independent experiments).