Fig 2: Intracellular CD3 marks thymus-dependent ILC precursors in peripheral tissues(A) Analyses of lung ILCs in indicated strains. The lineage cocktail and special efforts to exclude T and NK cells are described in the STAR Methods. ST2+ and ST2- subsets are color coded in blue and red. Bar graphs show cumulative data from at least three experiments. Unpaired Student's test was used for determining the statistical significance. *p<0.05, **<0.01, ***p<0.001, ****p<0.0001.(B) Analyses of small intestine ILCs in indicated strains. KLRG1+ and KLRG1- subsets are color coded in red and green. Bar graphs show cumulative data from at least three experiments. One-way ANOVA was used for determining the statistical significance. **<0.01, ***p<0.001, ****p<0.0001. Data are represented as mean ± SD.

Fig 3: Lin-CD127+icCD3e+ cells are distinct from conventional T cells(A) Detection of CD3e by intracellular and surface staining. Histograms show CD3e levels in the indicated subsets of cells in the indicated tissues.(B) Intracellular levels of TCRß or TCRd in icCD3e+ ILCs in comparison to T cells or Rag1-/- ILCs.(C) Quantification of the frequencies of icCD3e+ cells in Lin-CD127+ ST2+ and ST2- subsets in the lung of the indicated strain of mice.(D) Quantification of the frequencies of icCD3e+ cells in Lin-CD127+CD45hi KLRG1+ and KLRG1- subsets in the small intestine of the indicated strain of mice. *p<0.05, **<0.01, ***p<0.001. Data are represented as mean ± SD.