Fig 1: Interaction of miR-200a and its target genes. (A) CTNNB1, CDH1, PTEN, APC, CTNNA1 and SOD2 mRNA and protein expression levels were determined in KYSE150 ESCC cells following transfection with scramble control miRNA, miR-200a mimic or inhibitor using reverse transcription-quantitative PCR and western blot analysis. GAPDH was used as an internal loading control for both analyses. (B) Dual-luciferase assay using wild-type or mutated 3'-UTR sequences of target genes co-transfected with miR-200a mimics into ESCC cells. The cells were evaluated for luciferase activity following 48 h incubation. Data are presented as the mean ± SEM of three independent experiments. *P<0.05 and **P<0.01 vs. Scramble. APC, adenomatous polyposis coli protein; CDH1, cadherin-1; CTNNA1, catenin a1; CTNNB1, catenin ß1; ESCC, esophageal squamous cell carcinoma; SOD2, superoxide dismutase; UTR, untranslated region.