Fig 2: Overexpression of G3PP does not cause hypoglycemia in LS-Mpc2-/- mice in vivo.Male and female LS-Mpc2-/- and fl/fl mice were infected with adenoviral vector expressing the human G3PP or an empty control vector (1.5 x 109 PFU/mouse). (A) Representative western blot image for hepatic G3PP or vinculin protein abundance 4 or 7 days after adenoviral infection. (B) Fasting blood glucose and (C) blood lactate concentrations. (D) Plasma glycerol levels after a 5 h fast in the daytime. (E) Blood glucose concentrations after overnight fast and throughout an i.p. glycerol tolerance test. AUC, area under curve. Data are presented as mean ± SEM. Values are presented as mean ± SEM. For all panels, * indicates p-value = 0.05 versus mice administered empty viral vector.

Fig 3: Overexpression of G3PP impairs glycerol-mediated gluconeogenesis in vitro.Following infection with adenovirus expressing the human G3P phosphatase (G3PP) transgene or an empty control vector (1.5 x 109 PFU/mouse), primary hepatocytes from WT mice were isolated and plated. (A) Representative western blot image for G3PP and vinculin protein in isolated hepatocytes. (B-F) Cultured hepatocytes administered [U-13C3] glycerol in the presence or absence of UK5099 (5 µM). (B) Medium glucose concentrations measured by an enzymatic assay. (C-H) [U-13C3] Labeled incorporation into cellular (C) glycerol-3-phosphate, (D) DHAP, (G) glucose-6-phosphate or (H) glucose measured by mass spectrometry. Data are from a single experiment, triplicate per group. Values are presented as mean ± SEM. For all panels, * indicates p-value = 0.05 versus cells administered empty viral vector.