Fig 1: TETact activation of other genes in A20.a Expression of Cd3d, Cd3e, Cd3g and Cd8b in A20 SAM or TETact cells transduced with the promoter-targeting sgRNA. Expression level is relative to Actb as 2-?Ct. P = 0.0002 (Cd3d), 0.0002 (Cd3e), 0.0091 (Cd3g), 0.0391 (Cd8b) from one-way ANOVA with Tukey’s post hoc test. Data are shown as mean ± s.e.m. from three independent transductions. *P < 0.05, **P < 0.01, ***P < 0.001. b Bisulphite sequencing of Cd3d, Cd3e, Cd3g and Cd8b promoters for A20-TETact cells transduced with either control or the corresponding sgRNAs. Open lollipops indicate non-methylated CpG dinucleotides whereas closed lollipops represent methylated CpG motifs. Each row represents an individual clone. Five clones were analysed in each group. c Representative flow cytometry plots showing CD4 and CD8ß (proxy for CD8aß co-expression) surface expression in A20-TETact and DEADTETact cells transduced with vector co-expressing Cd4-, Cd8a- and Cd8b-targeting sgRNAs. Cells were assayed on day 7 post-sgRNA-transduction. Gates were drawn based on the negative population of cells transduced with control sgRNA in the same experiment. Source data are provided as a Source Data file.
Supplier Page from BioLegend for APC/Cyanine7 anti-mouse CD8b (Ly-3)