Fig 1: mRNA and total protein levels of occludin, tricellulin and ZO-1 upon exposure to each AHL in basal or inflammatory conditions. Caco-2/TC7 cells were treated on their apical side by each AHL (150 µM, 48 h) or on their basal side by IFN?+TNFa (50 ng/mL, 48 h). Treatments were performed individually or in combination as indicated. (a, b, c) mRNA levels were determined by (RT)-qPCR for occludin gene (OCLN) (a, ordinary one-way ANOVA ****P < .0001), tricellulin gene (MARVELD2) (b, Kruskall-Wallis test ****P < .0001) and Zonula Occludens-1 gene (TJP1) (c, ordinary one-way ANOVA P = .36). Results are expressed as 2-??Ct using cyclophylin as the house keeping gene (mean ± SEM of triplicates from 3 independent experiments). (d) Total protein levels of occludin, tricellulin and Zonula Occludens-1 and E-cadherin were quantified by Wes TM capillary electrophoresis. Reconstructed images are displayed, based on the area under the curve from chemiluminescence signal obtained for one experiment (representative of 3 independent experiments). Molecular weight markers (in kDa) are indicated on the left. (e) Total E-cadherin protein levels (Areas under the curve from chemiluminescence results, ordinary one-way ANOVA P = .82). (f, g, h) Total protein levels of occludin (f, ordinary one-way ANOVA ***P < .001), tricellulin (g, ordinary one-way ANOVA **P < .01), and ZO-1 (h, ordinary one-way ANOVA P = .8): areas under the curve from chemiluminescence results are normalized to respective E-Cadherin levels, which do not differ between conditions (e), and expressed as ratio values normalized to the control condition (mean ± SEM of duplicates from 3 independent experiments). For a-c and e-h, respective Holm-Sidak’s or Dunn’s posttests * P < .05 and ** P < .01 vs. control. C12 stands for 3-oxo-C12; C12:2 stands for 3-oxo-C12:2