Fig 1: MMP7 KD decreases drug efflux activity and metastatic potential of tumor cells. A, Cell morphology after transfection with MMP7 shRNA, GFP is used as a positive control. B, Quantitative PCR analysis of MMP7 expression in Parental and MMP7 KD cells. C‐E, RT‐PCR analysis of Hsp90α, Hsp90β, and MDR1 expressions in Parental and MMP7 KD cells, respectively. Note MMP7 KD decreasing the expression levels of Hsp90 and MDR1. F, Rh123 efflux activity assay showing decreased efflux activity in MMP7 KD cells compared to MMP7 OE cells. G, Trans‐well migration assay showing decreased invasive ability of MMP7 KD cells and is comparable with Parental cells which is furthered in response to 17AAG treatment. H, A statistical representation of G. I, Angiogenesis or tube formation assay of cells grown on 3D matrix. Note that MMP7 KD decreasing the number of branch points. K, A statistical representation of I. J, The in vitro scratch wound heal assay. Note that MMP7 KD decreasing the wound healing property of cells compared to Parental cells which is furthered in response to 17AAG treatment. L, A statistical representation of J. The values (mean ± SD) represented in figures, B, F, H, K, and L are average values obtained from three independent experiments. The significance values calculated from one‐way ANOVA and represented as, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001