Fig 1: LPS-stimulation enhances SP140 protein recruitment to TSS of TNF and IL1ß. (A) Protocol for an in vitro generation of DCs. Human primary CD14+ monocytes were incubated with 30 ng/mL GM-CSF and 20 ng/mL IL-4 for 5 days (D) to generate immature dendritic cells (iDCs). To generate mature DCs (mDCs), iDCs were washed with PBS and then stimulated with 100 ng/mL LPS. The illustration was created with BioRender.com. (B) SP140 gene expression was measured by qPCR in naïve iDCs or after 4 h of 100 ng/mL LPS-stimulation, from 3 individual donors (n = 3). (C) ChIP-qPCR of SP140 protein occupancy at TSS of TNF (n = 4) and (D) IL1ß (n = 3) in naïve iDCs or after 4, 6 or 24 h of 100 ng/mL LPS-stimulation, IgG was used as negative control. Statistical significance is indicated as follow: * p < 0.05 and ** p < 0.01. The error bars in a column graph represent standard deviation (SD).