Fig 1: Cx26 distribution at level of the cardiomyocyte cytoplasm. (a–c) Confocal laser scanning microscopy: representative images of rat and pig heart sections. Double immunofluorescence analysis for Cx26 (red) and Cx43 (green) show a distinct cell localization of the two Cxs. (a) Three dimensional picture of the maximum intensity projection of the raw images of longitudinal rat heart section treated with RaCx26-cl and MaCx43. Scale bar: 20 µm. (b) Blow up of the square shows section view after clipping of some planes. Arrows point out the clusters of Cx26 and arrowheads intercalated discs where Cx43 is mainly localized. Scale bar: 10 µm. (c) Three dimensional picture of the maximum intensity projection of the raw images of longitudinal pig heart section treated with RaCx26-Ct and MaCx43. Scale bar: 50 µm. (d–f) Immuno-electron microscopy: representative pictures of immunocytochemistry for Cx26 in rat ventricle samples. Scale bars: 200 nm. (d) Cx26 immune-gold particles (arrows) are localized at level of myofibrils (mf) and cytoplasmic vesicles (cv) but not at the intercalated discs (arrowheads). (e) Cx26 gold particles (arrows) label mithocondria (M) and myofibrils (mf). (f) Negative control does not show Cx26 immunolabeling.