Fig 2: SH Capsule Suppresses the EGFR Expression and ERK Phosphorylation in the Lung Tissues of LPS-Exposed Mice. (A) The expression of EGFR in the lung tissues was determined by western blotting. (B) ERK phosphorylation was examined in the lung tissues by western blotting. Data were presented as the mean ± SEM (n = 6). ## p < 0.01 vs. the untreated group. *p < 0.05, **p < 0.01 vs. LPS only treatment group.

Fig 3: Immunohistochemical staining for MAPK1 and NCOA2 (Original magnification×200).A, MAPK1 expression was found in cell cytoplasm and/or nucleus at various levels in HCC tissues; B, MAPK1 staining was negative in paracarcinomatous liver tissues; C, NCOA2 expression was found in nucleus of tumor cells at various levels in HCC tissues; D, NCOA2 staining was negative in paracarcinomatous liver tissues.

Fig 4: AGTR1 knockdown triggers cellular senescence of HCC cells in an ERK-dependent pathway. (A–E) p53 and p21 protein expressions in HepG2 along with Huh7 cell lines with sh-AGTR1 lentivirus transduction or ERK agonist EGF. (F–H) SA-ß-Gal activity of HCC cell lines with sh-AGTR1 lentivirus transduction or ERK agonist EGF. Scale bar, 10 µm. (I–K) SAHF activity of HCC cell lines with sh-AGTR1 lentivirus transduction or ERK agonist EGF. Scale bar, 10 µm.

Fig 5: Form regulates miR-149 and EphB3 expression and suppresses AKT/PI3K expression and STAT3 signaling in SW1116 and HCT116 cells. (A) SW1116, (B) HCT116 and (C) NCM460 cells were treated with Form (20, 50, and 100 µM) for 48 h, then miR-149 and EphB3 mRNA were determined using reverse transcription-quantitative polymerase chain reaction. Data are expressed as the mean ± standard deviation. *P<0.05 and **P<0.01 vs. control, n=5. (D) SW1116 and HCT116 cells were incubated with Form (100 µM) for 12 h, and the protein levels of p- and total PI3K, AKT, ERK, and STAT3 were determined using western blotting. The band intensities relative to AKT, PI3K, ERK and STAT3 are presented as the mean ± standard deviation. *P<0.05 vs. the control; n=6. Form, Formononetin; p-PI3K, phosphorylated-phosphoinositide 3 kinase; AKT, protein kinase B; ERK, extracellular regulated signal kinase; STAT3, signal transducer and activator of transcription 3; EphB3, Ephrin type-B receptor 3.