Fig 1: Gcc2-KO and Rab14-KO induce anti-tumor immunity.a B16 tumor growth in wild-type mice. Left, Western blot analysis of CRISPR/Cas9 knockout pooled B16 cells. Right, tumor volumes of wild-typepool, Sec24KO-pool, Gcc2KO-pool, Rab14KO-pool, or Npc1KO-pool B16 melanoma cells after subcutaneous injection in wild-type mice. b Wild-type, Gcc2KO and Rab14KO B16 cell proliferation analysis by IncuCyte. Representative single-cell CRISPR/Cas9 knockout clones are shown. c Wild-type, Gcc2KO and Rab14KO B16 tumor growth in wild-type mice. Left, mean tumor volume. Middle, individual mouse tumor growth curve. Right, Kaplan–Meier survival curve. d, e Wild-type, Gcc2KO and Rab14KO B16 tumor growth in Rag1-/- mice (d) and Ifnar1-/- mice (e). Left, mean tumor volume, Right, Kaplan–Meier survival curve. Data in b, c are representative of two independent experiments and in d, e are pooled from two independent experiments. Data (a–e) are shown as mean ± s.e.m. P values are determined by two-way ANOVA in (a–e). ns, not significant. Mantel-Cox tests were used for survival studies.