Fig 1: Combining profiles to determine the effect of pRB-depletion.a Relative RT-PCR levels of RB1, ARL14, SERPINA9, IL18, TPI, and PGK1 from RB-25/26 without DOX addition (WT) and plus DOX (RB-KD) (**p < 0.01, ***p < 0.001). n = 3 biological replicates and a total of 9 technical replicates. Error bars represent standard deviations from the replicates. b Western blots of pRB, WARS, ATP5E, ADP-GK and ACTIN from control and RB1−/− RPE1 cells. c Heat maps and statistical analysis of RNA and protein changes in pRB-depleted cells. d Correlations comparing RNA and protein changes between replicates, highlighting the poor correlations between RNA and protein.