Fig 2: Adenoviral STMN1 expression increases liver mass. (A) Gross images of representative mouse livers 9 days after injection with the adenovirus AdGFP or AdStath. Scale bar, 1 cm. (B) Liver weights and (C) liver to body weight ratios in uninjected control mice and mice at the indicated days after injection with AdGFP or AdStath (*P < 0.01, **P < 0.001, compared to AdGFP-infected mice at the same time point; n = 4-8). (D) Liver to body weight ratios at later times (*P < 0.01 compared to AdGFP-infected mice at the same time point; n = 4). (E) Relative hepatocyte cell size in adenovirus-infected cells (*P < 0.000001 compared to AdGFP-infected mice at the same time point; n = 340-890). Abbreviation: C/Con, control.

Fig 3: STMN1 phosphorylation depends on a functional FA pathway.Graphs displaying the ratio of total STMN1, pS16-STMN1 or pS38-STMN1 fluorescence intensity relative to ?-tubulin at centrosomes in FA patient-derived PD220 and PD20 cells ant their corrected counterparts PD220/A and PD20/D2 cells. Intensity of at least 20 mitotic cells were scored for each cell line from 3 separate experiments. P values are indicated in each graph.

Fig 4: AdStath infection increases hepatocyte STMN1 expression in mice. (A) Levels of Stmn1 mRNA relative to uninjected control liver in mouse livers at the indicated days after AdGFP or AdStath infection (*P < 0.05, **P < 0.01, # P < 0.001, compared to AdGFP‐infected mice at the same time point; n = 3‐6). (B,C) Immunoblots of total hepatic protein from uninfected mice (day 0) and mice infected with AdGFP or AdStath for the days indicated and probed for STMN1 and β‐actin. (D) Immunofluorescence images of mouse livers infected with AdGFP or AdStath for 3 and 5 days stained for STMN1 (original magnification ×400). Protein bands and molecular weights in kilodaltons are indicated by arrows in (B,C). Abbreviation: C/Con, control.

Fig 5: STMN1 phosphorylation requires FANCC.(A-C) FancC -/- and wild-type cells were immunostained with antibodies against total STMN1 (in A), STMN1-phospho-Serine 16 (in B), or STMN1-phospho-serine 38 (in C) along with ?-tubulin during mitosis and visualized using a Carl Zeiss Axio Imager M2 at 100X magnification. White line indicates scale of 1 µm. Ratio of pS16-STMN1, pS38-STMN1 or STMN1 fluorescence intensity relative to ?-tubulin at centrosomes were scored in at least 20 mitotic cells each from 3 separate experiments. P values are indicated in each graph.