Indirect immunostaining of PFA fixed rat hippocampus neurons (dilution 1 : 500).

Indirect immunostaining of pepsin treated mouse cerebellum sections (dilution 1 : 500). The animals were perfusion fixed with 4 % formaldehyde and 15 % picric acid (Danbolt et al. 1998, Methods in Enzymology 296: 388-407.)

Courtesy: Z. Nusser, Laboratory of Cellular Neurophysiology, Institute of Experimental Medicine, Budapest.

Indirect immunolabeling of PFA fixed, paraffin embedded mouse cerebellum with guinea pig anti-GAT 1 (dilution 1 : 500; red) and chicken anti-calbindin (cat. no. 214 006, dilution 1 : 500; green). Nuclei have been visualized by DAPI staining (blue).