Fig 1: Characterization of IN:V151I Effect on HIV Replication: (A) Comparison of HIV replication capability. PHA-stimulated primary CD4(+) T cells from healthy donors were infected with 10 ng p24 amounts of HIV(WT) or variants containing mutation V151I, M50I, and M50I/V151I. The infected cells were cultured for 14 days with media changed every 3–4 days. HIV replication was monitored using a p24 antigen capture kit (PerkinElmer). Representative data from three independent assays are presented as mean ± SD (n = 3). (B) A total of 1 µg of total viral particle proteins from HIV(WT) and HIV variants were subjected for WB using polyclonal anti-Anti-HIV1 p55 (Gag)+p24(CA)+p17(MA) antibody (Cat# ab63917, Abcam) as described in the Materials and Methods.