Fig 2: Exposure to cryptic collagen IV significantly increases migration and adhesion potentially via activations of DDR1 and AKT in K562 cells. (A) Transwell migration of K562 cells exposed to either soluble native or heat-denatured collagen IV after 4 h. (B) Cell adhesion assays were performed in K562 cells upon exposure to native or denatured collagen IV. (C) DDR1 phosphorylation levels were examined using PathScan pDDR1 (panTyr) Sandwich ELISA upon exposure to either soluble native or heat-denatured collagen IV. Total DDR1 was assessed in the ELISA lysates (1 = Native Collagen IV, 2 = Denatured Collagen IV). (D) Activation of AKT, STAT5 and MAPK was examined via phosphoblotting. Steady-state levels including ß-tubulin levels were also assessed (left panel). Phosphoblotting was quantitated using Image J and normalized to total protein and ß-tubulin to calculate the ratio of phosphorylation between denatured and native collagen exposure (right panel). (E) DDR1 expression was knockdown using siRNA for DDR1. Activation of AKT was examined using phosphoblotting. Total protein levels including ß-tubulin were also assessed.