Fig 1: IQGAP 1 location regulates cortactin-associated formation of filopodia and lamellipodia.A Representative images of MF and IQGAP1 or IQGAP2 distribution in the directional migration model or after CXCL12 treatment. (green, FITC-stained MFs; red, TRITC-stained IQGAP1/IQGAP2), scale bar, 10 µm. B Representative images of MF and IQGAP1 or IQGAP2 distribution in the wound healing assay. (green, FITC-stained MFs; red, TRITC-stained IQGAP1/IQGAP2), scale bar, 10 µm. C Western blotting analysis of Cortactin, IQGAP1, and IQGAP2 levels in the NC, IQGAP1-siRNA, IQGAP2-siRNA, and IQGAP2 and IQGAP2 rescue groups. D Western blotting analysis of cortactin levels in the WT, S311A, S311D, S-A, and S-D groups. E Western blotting analysis of cortactin levels in the control, NC, RSK- siRNA, and RSK inhibitor treatment groups. F Representative images of filopodia and lamellipodia structures in the Clip170 mutation, and NC, IQGAP1 and IQGAP2-siRNA groups (scale bar, 10 µm, green, FITC-stained MFs; blue, nucleus; white arrows: filopodia and lamellipodia structures).
Fig 2: IQGAP1 and 2 are correlated in Clip170 tension magnitude and distribution.A Representative images of MDA-MB-468 expressing the Clip170-cpstFRET probe in the IQGAP1-siRNA, IQGAP2-siRNA, IQGAP2 overexpression, and IQGAP2 rescue groups. B Normalized CFP/FRET signals, comet lengths, comet quantities, and fluorescence intensities for the whole cell in the different groups (scale bar, 10 μm, n ≥ 6 cells in each group, ***P < 0.001 compared with NC group). C Representative images of MDA-MB-468 cells expressing the Clip170-cpstFRET probe in the IQGAP1-siRNA, IQGAP2-siRNA, IQGAP2 overexpression, and IQGAP2 rescue groups in the directional migration model, scale bar, 10 μm.
Supplier Page from Proteintech Group Inc for IQGAP2 antibody