Fig 2: Immunolocalization of BDNF+ cells in the cerebral cortex of rats. Scale bar: 50 μM. (A) Staining area of the BDNF+ cells in the cerebral cortex. (B) Data are mean ± SEM, n = 7/group, * p < 0.05, ** p < 0.01, and *** p < 0.001 (one-way ANOVA, Tukey’s post-test study). (C) Western blot analysis of BDNF and NAAA/ASAHL levels in primary culture of astroglia. * p < 0.05, Significant differences between the “control” and “Syn” groups. Data are presented as mean ± SEM, n = 9 (number of samples analyzed), (pairwise comparison, Student’s t-test). Data normalized to α-tubulin.

Fig 3: Western blot analysis of the levels, IL-1β, IL-6, and TNFα in RAW264.7 macrophage lysates (a,c), expression of ASAHL protein in cell culture after the addition of OEA-DS at concentrations of 1 and 10 μg/mL (b,d). Expression levels are normalized to α-tubulin and expressed relative to controls. For the ASAHL, data are mean ± SEM, n = 6/group, * p < 0.05 (one-way ANOVA, post hoc Tukey test). For the cytokines, data are mean ± SEM, n = 4 (number of repetitions of the experiment), * p < 0.05 versus “CTL” + p < 0.05 versus “LPS” (Student’s test).