Fig 1: Characterization of IBI104 in the in vitro cell-based assays. (A, B) In an MLR assay, matured DCs and CD4 T cells were co-cultured in different concentrations of antibodies for 96 h. (A) IL-2 and (B) IFN? were analyzed using ELISA. (C, D) In an NK activation assay, NK and K562 cells were co-cultured; Graphs show different concentrations of antibodies for 4 h. (C) NKG2D and (D) CD107a were analyzed using flow cytometry. (E) In the TIM-3 internalization assay, pre-activated T cells were plated onto a flat-bottom dish for 3 days at 37°C; cell suspension included Fab-ZAP along with mAb. The determination of cell viabilities was performed using the Cell Counting Kit-8. All experiments were repeated in at least two independent sets showing representative results. (F) TIM-3 internalization assay in pre-activated T cells was performed using IBI104 and TSR-022 monoclonal antibodies for 3 days as in (E).