Fig 2: MALAT1 mediates PSF interaction with several hnRNP proteins other than PTBP1.(A) Native RIP followed by RT-qPCR detecting MALAT1 and 7SK RNA retrieved by hnRNP A1–, hnRNP F–, or hnRNP U–specific Ab or by normal IgG in HEK293 cells. Data are shown as means ± SD of n = 3 independent experiments. (B) Immunoblotting using the indicated Abs detecting IP efficiency of the native RIP assay in (A). (C) Co-IP assay with anti-PSF Ab detecting the effect of RNase T1 treatment, DNase I treatment, or MALAT1 knockdown on PSF interaction with the indicated hnRNP proteins in HEK293 cells. The dose of RNase T1 and DNase I was 15 U per reaction.