Fig 1: (A) GRM8, AP1S2, and MFAP2 mRNA levels of two normal brain tissue obtained from epilepsy surgery (Numbered 1 and 2), three Wingless/Sonic Hedgehog medulloblastoma (MB) (Numbered 3–5), and three Group 3 MB (Numbered 6–8). H-actin was used as the endogenous reference gene. The expression level is presented with graphs. (B) Heatmap of Immunohistochemistry staining with (GRM8 encoding), (AP1S2 encoding), and (MFAP2 encoding) antibodies for the 33 Group 3 MB. Protein IOD/Are is represented by the indicated color. (C) Images of immunohistochemistry staining showing high and low expression of MGLUR8 (GRM8 encoding), AP1S2 (AP1S2 encoding), and MAGP1 (MFAP2 encoding) in the series of Group 3 MB patients (10X and 40X magnification). Kaplan-Meier survival curve of the 33 patients with Group 3 MB grouped by expression level of GRM8, AP1S2, and MFAP2 encoding. Data are mean ± s.e.m. ****P < 0.0001; NS, not significant (P > 0.05).
Fig 2: (A) Diagram of the screen process of Kaplan-Meier survival and variation analyses for the marker genes of cluster 6 with two cohorts of microarray data. Fifty-four genes showed specific upregulation in one tumor type or normal brain. Twenty genes exhibited correlation with the survival of patients with medulloblastoma (MB). Ten genes contained significant differences of either expression or survival analysis, including TUBB4A, TSHZ1, SLITRK1, POU4F1, MPHOSPH6, MFAP2, KIF5C, GRM8, CCND2, and AP1S2. (B) Box plot of variant analysis of microarray data of the MB patient cohort grouped showing expression levels of TUBB4A, TSHZ1, SLITRK1, POU4F1, MPHOSPH6, MFAP2, KIF5C, GRM8, CCND2, and AP1S2 in various brain tumors and normal brain. NT, normal tissue; MB, medulloblastoma; EM, ependymoma; Pa, pilocytic astrocytoma; GBM, glioblastoma. (C) Kaplan-Meier survival curve of microarray data of the MB patient cohort grouped by TUBB4A, TSHZ1, SLITRK1, POU4F1, MPHOSPH6, MFAP2, KIF5C, GRM8, CCND2, and AP1S2 expression level. Data are mean ± s.e.m. *P = 0.05; **P = 0.01; ***P = 0.001; ****P < 0.0001; NS, not significant (P > 0.05).
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