Fig 1: Immunoblot detection of proteins in HDL isolates after sPLA2 treatment.Serum was treated with 0.2 µg/ml sPLA2 overnight to increase the content of free fatty acids and lysophosphatidylcholine. Afterwards, HDL was isolated by density gradient ultracentrifugation and the protein composition of the preparation was assessed by Western blot. (A) HDL preparations were separated by SDS-Page, blotted and probed using specific antibodies for apoA-IV, a-1-antitrypsin (A1AT) or human serum albumin (HSA). Shown is a representative example of one preparation run in quadruplicate. (B) Blots were imaged on a Chemidoc Imaging machine and analyzed with ImageLab 5.2. Results represent the combined analysis of 3 independent HDL preparations run in quadruplicates on SDS Page gels. *p < 0.05 vs. control.