Fig 2: Knockdown of HTATIP2 partially reverses NONHAT076754 silencer-induced repressive effects on EOC metastasis. A. Knockdown of HTATIP2 partially reversed NONHAT076754 silencer-induced inhibition of migration in SKOV3 detected by wound healing assays. B. Knockdown of HTATIP2 partially reversed NONHAT076754 silencer-induced inhibition of migration in OVCAR5 cells detected by wound healing assays. C. Transwell migration assays confirmed the rescue action of HTATIP2 interference on altered migration activity caused by NONHAT076754-siRNA. D. Knockdown of HTATIP2 partially reversed NONHAT076754 silencer-generated anti-invasion in SKOV3 and OVCAR5 cells determined by transwell invasion assays. Data are shown as the mean± SD. * p < 0.05, ** p < 0.01 vs. NC groups.

Fig 3: Interrogation of growth signaling in hearts of TIP30-deficient mice during cardiac hypertrophy A, BWestern blot analysis of indicated proteins in hearts from TIP30 WT and Het mice 6 weeks after TAC or sham surgery (A) and their quantification (B). N = 3-4 mice/group.Data information: Data are shown as mean ± SEM. *P < 0.05, **P < 0.01, ****P < 0.0001. One-way ANOVA with Sidak's multiple comparisons test.Source data are available online for this figure.

Fig 4: TIP30 inhibits cardiomyocyte protein synthesis at the level of translational elongation ASchematic representation of polysome profiling.BRepresentative polysome profiles from NRCM after adenoviral transduction. Western blot analysis of rpS6 is shown to determine 40S, 60S, and 80S monosome fractions.C–ESchematic representation of the dual-luciferase reporter assay (C). Quantification of renilla (D) and firefly (E) luciferase activity in neonatal rat cardiomyocytes (NRCM) after adenoviral transduction either with control virus (Ad. Con) or Ad.TIP30, stimulation with phenylephrine (PE) as indicated and transfection with the bi-cistronic reporter construct, as shown in (C) (N = 7–8 samples/group). *P < 0.05, **P < 0.01. One-way ANOVA with Sidak's multiple comparisons test.F, GWestern blot analysis of puromycin incorporation in hearts 6 weeks after TAC or sham surgery in AAV-Con or AAV-TIP30-treated C57BL/6 WT mice and their quantification (N = 3–7 mice/group). All lanes were run on the same gel, but were noncontiguous as indicated by separate boxes. Puromycin was injected 3 h prior to sacrifice. *P < 0.05, **P < 0.01. One-way ANOVA with Sidak's multiple comparisons test.Data information: Data are shown as mean ± SEM.Source data are available online for this figure.

Fig 5: TIP30 deficiency results in enhanced cardiac hypertrophy during pathological overload ASchematic representation of the study design and Western blot analysis for TIP30 and GAPDH in hearts from TIP30 wild-type (WT), heterozygous (Het), and homozygous knock-out (KO) mice under basal conditions.B–IQuantification of heart weight (HW)/tibia length (TL) ratio (B), lung weight (LuW/TL) ratio (C), echocardiographic fractional area change (D) and left ventricular end-diastolic area (LVEDA; E), dP/dt max and dP/dt min (Millar catheter; F, G), and a-MHC and ß-MHC transcript abundance (H, I). N = 4–18 mice/group, all 6 weeks after TAC or sham surgery. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001. One-way ANOVA with Sidak's multiple comparisons test.J, KRepresentative images of Sirius red-stained heart sections (scale bar: 1 mm) (J) and fibrosis quantification (K) of indicated mice 6 weeks after TAC or sham surgery. N = 3-5 mice/group.LQuantification of cardiomyocyte area of isolated adult cardiac myocytes (N = 4–11 mice/group) of indicated mice 6 weeks after TAC or sham surgery. *P < 0.05. One-way ANOVA with Sidak's multiple comparisons test.M, NMicroscopy images of heart sections of indicated mice 6 weeks after TAC surgery stained for isolectin B4 (green) and WGA (red, M) and quantification of capillaries per myocyte (N). (N = 5–7 mice/group, scale bar: 50 µm). **P < 0.01. One-way ANOVA with Sidak's multiple comparisons test.OSchematic representation of AAV-TopT-TIP30 study design.PWestern blot analysis for TIP30 and GAPDH in hearts from TIP30 wild-type (WT) and heterozygous (Het) after AAV-TropT-TIP30 or AAV-control (AAV-Con) injection followed by 6 weeks of TAC surgery.Q, RQuantification of HW/TL ratio (Q) and LuW/TL (R) ratio in AAV-Con or AAV-TropT-TIP30 treated Tip30 heterozygous (Het) or WT mice 6 weeks after TAC or sham surgery (N = 5–11 mice/group). *P < 0.05, ***P < 0.001. One-way ANOVA with Sidak's multiple comparisons test.Data information: Data are shown as mean ± SEM.Source data are available online for this figure.