Fig 1: VEEV interaction with RNAi pathway. (A) 293T cells were infected with VEEV TC-83 at an MOI of 1.0 and lysates were collected at 18 hpi. Lysates were immunoprecipitated using anti-Martin 3, anti-nsP2 and IgG antibodies, and analyzed by Western blot to determine interaction with nsP2. (B) Schematic representation of steps involved to knockdown RNAi pathway protein. (C,D) 293T cells were transfected with siRNAs for Ago2, Dicer, MOV10, TRBP2 or Martin 3. (C) Cells were infected with TC-83 at an MOI of 0.01 and cell supernatants were collected at 8 and 24 hpi. (D) Cells were infected with VEEV TC-83 at an MOI of 0.1 and cell supernatants were collected at 8 and 24 hpi. Viral titer was determined via plaque assay and negative siRNA was used as a control. Results are representative of three independent experiments (n = 3). * p < 0.0332, ** p < 0.0021, *** p < 0.0002.