Fig 1: Exacerbation of oxidative stress and downregulation of protein kinase G signaling in the left ventricle of ucp3+/- rats during hypertension. A, Quantification of 4-hydroxynonenal protein adducts, (B) total protein sulfenation amounts, (C) phosphorylation levels of cardiac troponin I at Ser23/24 and Ser150, and (D) phosphorylation levels of vasodilator-stimulated phosphoprotein at Ser239 in the left ventricle of ucp3+/- and ucp3+/+ rats after 4 weeks of a combination treatment with high dietary salt intake and Ang II infusion. Control animals were fed a low-salt diet and infused with saline. Protein amounts were normalized to HSP60 (heat shock protein 60) or cardiac troponin I protein levels. Data are expressed as mean±SEM. Data were analyzed by 2-way ANOVA with Tukey test. cTnI indicates cardiac troponin I; HS/Ang II, high dietary salt intake and Ang II infusion; HSP60, heat shock protein 60; LS/Saline, low-salt diet and infused with saline; p-cTnI, phospho-cardiac troponin I; p-VASP, phospho-vasodilator-stimulated phosphoprotein; and UCP3, uncoupling protein 3. P<0.05 vs *LS/Saline ucp3+/+, †LS/Saline ucp3+/-, and ‡HS/Ang II ucp3+/+.
Supplier Page from Abcam for Anti-Cardiac Troponin I (phospho S150) antibody