Fig 1: Clodronate promoted the transplantation of iCell-MG in slice cultures. (A) Experimental paradigm. (B) Representative images of iCell-MG-transplanted slices at 24 DIV cultured with (lower) and without clodronate (upper, control). Cultures were immunostained for Iba1 (green) and human nuclei (red). Nuclei were stained with Hoechst (blue). (C) Magnified images of control (upper panels) and clodronate-treated slices (lower panels) at 24 DIV. (D) iCell-MG density at 17 (left panel), 21 (middle panel), and 24 DIV (right panel). ***p < 0.001 and*p < 0.05, Student’s t-test, n = 6 (17 DIV in control), four (17 DIV in clodronate), four (21 DIV in control), four (21 DIV in clodronate), four (24 DIV in control), and six slices (24 DIV in clodronate), each from two mice. Data represent the mean ± SD. (E) Replacement rate of iCell-MG at 17 (left panel), 21 (middle panel), and 24 DIV (right panel). *p < 0.05 and***p < 0.001, Student’s t-test, n = 4–6 slices. Data represent the mean ± SD. (F) Representative images of innate and iCell microglial distribution at 24 DIV cultured with (lower) and without clodronate (upper, control). iCell-MG were distributed from the surface to near the bottom of slices (arrows). (G) Representative images of innate and iCell microglia at 24 DIV with clodronate. Cultures were immunostained for Iba1(green), mouse TMEM119 (mTMEM119; blue), and human TMEM119 (hTMEM119; red). (H) Representative images of innate and iCell microglia at 24 DIV with clodronate. Cultures were immunostained for Iba1 (green), CD68 (blue), and human nuclei (red). (I) Representative images of innate and iCell microglia at 24 DIV with clodronate. Cultures were immunostained for Iba1 (green), human P2RY12 (hP2RY12; blue), and human nuclei (red).