Fig 1: Sap-B, the cleavage product of PSAP, is increased in mitochondrial isolates. A) Schematic diagram of the protein PSAP with its 4 daughter proteins, peptides identified from proteomic analyses, and targeting of the polyclonal antibody used in the present study. B) Western blot analysis of ?H2AX in nuclear fractions and PSAP, SAP-B, and mitochondrial 70-kDa heat shock protein (mtHSP70) in mitochondrial fractions of MSCs irradiated or not with 2 Gy X-rays for 4 h. C) Quantification of samples analyzed in B (means ± sem of 5 independent experiments). Ctrl, control. A Student’s unpaired t test was performed. *P < 0.05.
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