Fig 1: Validation of selected ABC genes by qPCR. (A) ABCA1; (B) ABCA6; (C) ABCA9; (D) ABCB1 (E); ABCB11; (F) ABCC3; and (G) ABCG4 mRNA expression, comparing first trimester (n = 8) and term (n = 12) placentae. Statistical analyses: non‐parametric Mann‐Whitney test. Data are presented as mean ± SEM. **P < 0.01 and ***P < 0.001
Fig 2: Human placental ABCA6 protein expression and immunolocalization towards term. (A) Representative ABCA6 (180 kDa) and ERK (42 kDa) immunoblots, comparing first trimester (n = 5; open bar) and term (n = 5; solid bar) human placentae, as well as placental ABCA6 expression normalized to ERK (loading control). (B and C) Representative images of ABCA6 localization in first trimester and term placentae, respectively. (D and E) Negative controls for the respective gestational age. Statistical analyses: unpaired t‐test. Data are presented as mean ± SEM *P < 0.05, arrows indicate the syncytium, scale bar: 55 μm
Supplier Page from Abcam for Anti-ABCA6 antibody