Fig 1: BAG5 is a target of miR-142-5p. (A) BAG5 targeted by miR-142-5p. (B) Expression of BAG5 in cells transfected with pc-BAG5 was significantly upregulated compared with BAG5 NC. (C) mRNA expression level of BAG5. (D) Protein expression level of BAG5. (E) Signal density of BAG5 was reduced after treatment of miR-142-5p mimics, compared with miR-142-5p mimics NC group. *P<0.05, **P<0.01 vs. miR-142-5p mimics NC, ##P<0.01 vs. BAG5 NC. NC, negative control; miR, microRNA; UTR, untranslated region; MUT, mutant; WT, wild-type; BAG5, Bcl-2 associated athanogene 5.
Fig 2: BAG5 regulates the cytokines. Levels of TNF-a, IL-1ß, IL-6 and IL-8. **P<0.01 and ***P<0.001 vs. LPS. #P<0.05 and ##P<0.01 vs. LPS + pc-BAG5 group. LPS, lipopolysaccharide; NC, negative control; miR, microRNA; BAG5, Bcl-2 associated athanogene 5; ns, not significant.
Fig 3: BAG5 regulates the proliferation and apoptosis of LPS-treated MAC-T cells. (A) Proliferation of MAC-T cells was examined by performing the clone formation assay. (B) Apoptosis of MAC-T cells. (C) Expression of cleaved-Caspase-3, cleaved-Caspase-9, Bcl-2 and Bax proteins. **P<0.01 vs. LPS. #P<0.05 and ##P<0.01 vs. LPS + pc-BAG5 group. LPS, lipopolysaccharide; NC, negative control; miR, microRNA; BAG5, Bcl-2 associated athanogene 5; ns, not significant.
Fig 4: BAG5 regulates the cell viability of LPS-treated MAC-T cells. (A) mRNA expression levels of BAG5. (B) Protein expression levels of BAG5. (C) Signal density of BAG5 (magnification, x200). (D) Cell viability of MAC-T cells. **P<0.01 and ***P<0.001 vs. LPS. #P<0.05 and ##P<0.01 vs. LPS + pc-BAG5 group. LPS, lipopolysaccharide; NC, negative control; miR, microRNA; BAG5, Bcl-2 associated athanogene 5; OD, optical density.
Supplier Page from Abcam for Anti-Bag5 antibody