Fig 1: Knock-down of CXCL11 affects MM cells proliferation, apoptosis and macrophages M2-like polarization in vitro. A The mRNA expression of CXCL11 in MM cell lines. B CCK-8 assays revealed that CXCL11 downregulation decreased MM cell proliferation. C, D. Apoptosis analysis by flow cytometry in U266 and RPMI 8226 cell lines (C.U266; D.RPMI 8226). E CD206 protein expression in macrophages was analyzed by western blot assay at 72 h following co-culture. F The expression of M1 and M2 polarization-related markers in macrophages co-cultured with U266 and RPMI 8226 cells transfected with sh-CXCL11 or control was detected by qRT-PCR. Data were presented as the mean ± SD from three independent experiments. *p < 0.05; **p < 0.01, ***p < 0.001
Fig 2: Downregulation of CXCL11 suppressed tumorigenesis in vivo. A Representative images of tumors removed from the mice. B Body weight and tumor volumes in two groups were observed on the indicated days and tumor weight were shown after removed. C Flow cytometry to detect the expression of CD206 in macrophages in primary tumors in the two groups. D IHC analysis of CXCL11 and CD206 in primary tumors of two groups. Data were presented as the mean ± SD from three independent experiments. *p < 0.05; **p < 0.01
Fig 3: Analyses of immune cell infiltration. A Correlations of IRGPI with immune cell infiltration (The blue and red violin represented the IRGPI LR and HR group, respectively. The white points inside the violin implicated median values). B, C Significant correlations of 3-IRGs (CD70, CXCL11, HGF) with M0 macrophages, M1 macrophages and M2 macrophages in MM. D A brief process for M2 macrophage induction. The CD206 expression of M2 macrophages was determined by flow Cytometry (E) and Western blot analysis (F). G qRT-PCR analysis of 3-IRGs after MM cells co-cultured with M2 macrophages. Data were presented as the mean ± SD from three independent experiments. *p < 0.05; **p < 0.01, ***p < 0.001
Fig 4: CXCL11 expression is associated with prognosis of MM. A Western blot analysis, qRT-PCR analysis (B) of CXCL11 expression in MM tissues and normal control. C The CXCL11 expression varies with the stage of the patient. DThe relationship between the expression of overall survival and CXCL11. E The images of immunohistochemistry for MM and normal tissues. (The date in C, D were collected from GSE136324). *p < 0.05; **p < 0.01, ***p < 0.001
Supplier Page from Abcam for Anti-CXCL11 antibody [EPR11076]