Fig 2: Time course of the phosphorylation of JAK2, STAT5, AKT, mTOR, MEK1/2, and ERK1/2 and the quantitative variation of PRLR and ER-a in Ishikawa cells after adding prolactin.(a) The maximal phosphorylation of JAK2 was observed in Ishikawa cells 1 hour after treatment with 100 ng/ml of prolactin.(b) The phosphorylation of STAT5 was not changed in Ishikawa cells after treatment with 100 ng/ml of prolactin.(c) The phosphorylation of AKT and mTOR was not changed in Ishikawa cells after treatment with 100 ng/ml of prolactin.(d) The maximal phosphorylation of MEK1/2 and ERK1/2 was observed in Ishikawa cells 5 minutes and 15 minutes after treatment with 100 ng/ml of prolactin, respectively.(e) The increased expression of PRLR of 110 kDa and ER-a was observed in Ishikawa cells 6 hours after treatment with 100 ng/ml of prolactin(f) The maximal phosphorylation of ERK1/2 was observed in Ishikawa cells 5 minutes and 15 minutes after treatment with 100 ng/ml of prolactin, and the phosphorylation of ERK1/2 was completely inhibited in Ishikawa cells pretreated with 10 µM of U0126 after treatment with 100 ng/ml of prolactin.(g) The increased expression of PRLR of 110 kDa and ER-a was observed in Ishikawa cells 6 hours after treatment with 100 ng/ml of prolactin, and the decreased expression of PRLR and ER-a was observed in Ishikawa cells pretreated with 10 µM of U0126 6 hours after treatment with 100 ng/ml of prolactin.