Fig 1: A proposed model illustrating mechanism of ribosome recruitment on the XIAP IRES. The scaffolding protein factor eIF3 binds directly to the XIAP IRES in an RNA conformation dependent manner. The interaction of eIF3 with PABP, likely via PAIP 1, enables RNA circularization. This RNP complex subsequently facilitates ribosome recruitment to the XIAP IRES RNA in the vicinity of AUG.
Fig 2: Effect of PAIP1 Knockdown on HCC Cell Apoptosis and Colony Formation. A, B Annexin V/PI-FACS analysis of apoptosis levels in A SMMC-7721 and B HEPG2 cells. C, D Assessment of colony formation ability in C SMMC-7721 and D HEPG2 cells. Cells were plated in a six-well plate (500 cells/well) for a period of 10 days. Clones were counted after staining with Giemsa. Results are displayed as means and standard deviations (SDs) from three independent experiments. *P < 0.05, **P < 0.01
Fig 3: Effect of PAIP1 Knockdown on In Vivo SMMC-7721 Xenograft Tumorigenicity. A SMMC-7721 cells were subcutaneously (s.c.) injected into the dorsal flanks of nude mice. B Graph of xenograft tumor volumes over the initial 17-day period. C Final tumor weights were measured 28 days after s.c. injection. NC negative control tumor group, KD PAIP1-knockdown tumor group. *P < 0.05, **P < 0.01 means significant difference between NC and KD group from the beginning day
Fig 4: PAIP1 Upregulation in HCC Cell Lines and PAIP1 Knockdown by siRNA. A Real-time polymerase chain reaction (RT-PCR) and B Western blotting were used to determine PAIP1 expression in the four HCC cell lines relative to that of the normal hepatocyte cell line L02. C RT-PCR and D Western blotting validated siRNA-mediated PAIP1 knockdown in the SMMC-7721 cell line. The HEPG2 cell line displayed similar siRNA-mediated PAIP1 knockdown (data not shown). GAPDH served as the loading control in all experiments. Results are displayed as means and standard deviations (SDs) from three independent experiments. **P < 0.01
Fig 5: Cyclin D Pathway Dysregulation Induced by PAIP1 Knockdown. A Cluster analysis of miRNA expression in PAIP1-knockdown (KD) and negative control (NC) SMMC-7721 xenograft tumor cells. Each row represents a unique miRNA, and each column represents an individual tumor sample (n = 3 tumors in each group). Red or green coloring represents elevated or decreased expression, respectively. B Results of the KEGG pathway analysis showing the top ten highest-ranking KEGG pathways. The ‘focal adhesion’ and ‘pathways in cancer’ pathways have been circled in red. C Representative Western blots of four key cyclin D pathway genes. GAPDH served as loading control. D The relative expression of each protein is graphically displayed. Results are displayed as means and standard deviations (SDs) from three independent experiments. *P < 0.05, **P < 0.01
Supplier Page from Abcam for Anti-PAIP1 antibody [EPR13258(B)]