Fig 1: Cross-talk between mitochondria and ER/SR facilitates mitochondrial calcium cycling.a Representative co-immunoprecipitation image from a cardiac ventricular sample of 8-week-old mice (n = 3) in WT and KO (Input—non-IP sample; IgG—control antibody; IP—VDAC2 protein pull-down); b Representative proximity ligation assay image for VDAC2–SERCA2 and VDAC2–NCX1 interaction in 8-week-old mice (scale bar, 10 µm) (n = 3); c Representative immunofluorescence image from 16-week-old mice heart (scale bar, 10 µm) (n = 3).
Fig 2: NCX1 expression was increased in the prostate cancer tissues and KB-R7943 induced autophagosome accumulation in prostate cancer cells(A) Results of the immunohistochemistry assay in prostate tissues. (B) Western blots conducted in prostate cancer cell lines. (C) Differences in protein levels between benign and malignant prostate tissues based on integral optical density analysis. (D) Differences between benign and malignant prostate tissues with different Gleason Scores. (E and F) PC3 cells were treated with the indicated concentrations of KB-R7943 for 24 h or with 30 µM KB-R7943 for the indicated periods of time, and LC3-I to LC3-II transition was analyzed using Western blotting. (G) PC3 cells stably expressing eGFP-LC3 (green) were treated with full or serum-starved medium or with 30 µM KB-R7943 for 24 h and examined in an immunofluorescence assay. Numbers of GFP-positive puncta per cell (n = 50) from three independent experiments are shown. (H) PC3 cells were treated with full medium, serum-starved medium, or 30 µM KB-R7943 for 24 h and examined using transmission electron microscopy analysis. Typical double-layer membrane autophagosomes (black arrows) are shown. The data are shown as means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.
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