Fig 1: Dose–response and time effects of curcumin on the mRNA expression of SREBP-2, SP-1, and SCAP. (a) mRNA expression at 24 and 48 hr cultivation in different concentrations of curcumin *, p < .05, versus the untreated control. (b) mRNA expression under 25 µM curcumin treatment from 0 ~ 96 hr *, p < .05, versus the untreated control. (c) mRNA expression under 25 µM curcumin treatment from 0 ~ 12h.*, p < .05, versus the untreated control. (d) The comparison of mRNA expression in control group and curcumin group at each time point. *, p < .05, versus the untreated control in each point-in-time. Sketch Map. Normally, INSIG binds to the sterol-sensing domain (SSD) of SCAP (blue transmembrane region in SCAP). As the sterols concentration decreases, the connection between SCAP and INSIG disappears, and the complex of SREBP/SCAP is transported from the endoplasmic reticulum to the Golgi apparatus. S1P and S2P were sequentially involved in cleaving SREBP-2 and releasing the mSREBP-2 into the cell nucleus
Fig 2: The expression of S1P/S2P mRNA and protein under 25 µM curcumin treatment. (a) The change of mRNA expression. *, p < .05, versus the untreated control (first column). (b) The Western blot and the gray value analysis results. ß-actin was used as an invariant control for equal loading. *, p < .05, versus the untreated control (first column)
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