Fig 1: Role of mitophagy in the protective effect of PD against sepsis-induced NLRP3 activation. The mice were subjected to CLP and administered vehicle, PD (30 mg/kg), or PD along with mdivi-1 (3 mg/kg). Parkin−/− mice were subjected to CLP and administered PD (30 mg/kg). The mice were sacrificed at 12 h after CLP. a The expression of NLRP3, ASC, pro-caspase-1, caspase-1 p10, pro-IL-1 and IL-1β was measured by western blot analysis. Quantification of b NLRP3, c ASC, d pro-caspase-1, e caspase-1 p10, f pro-IL-1, and g IL-1β. h IL-1β in the kidney was measured by ELISA
Fig 2: Protein levels of NLR family pyrin domain containing 3 (NLRP3), apoptosis-related spot-like protein (ASC), cleaved caspase-1 and caspase-1 in perivascular adipose tissue from Zucker lean rats (controls) and male Zucker diabetic fatty rats, with or without liraglutide for 12 weeks (DM and DM+GLP-1 groups): (a) representative western blots; (b) NLRP3 and ASC levels normalised to β-actin; and (C) ratio of cleaved caspase-1/caspase-1. Data presented as mean ± SEM of 10 rats per group (samples assayed in triplicate); *P < 0.05, DM versus controls, or DM+GLP-1 versus DM group (analysis of variance).
Fig 3: The expression differences of key molecules in NLRP3 inflammasome-mediated pyrolysis (NLRP3, ASC, caspase-1, IL-1β, and GSDMD) in mRNA level (A) and in protein level (B) in the control, cisplatin, cisplatin+siNC, and cisplatin+siNLRP3 groups. (C) The difference in the IL-1β concentrations in the supernatant of each group. Representative results of at least three repeated experiments are shown. *p < 0.05, **p < 0.01, ***p < 0.001. ns, no significance.
Fig 4: BJJ downregulated NF-κB, TXNIP, NLRP3, ASC, caspase-1, and IL-1β mRNA expression in diabetic ApoE−/− mouse aortas. (a) NF-κB mRNA level determined by rRT-PCR. (b) TXNIP mRNA. (c) NLRP3 mRNA. (d) ASC mRNA. (e) Caspase-1 mRNA. (f) IL-1β mRNA. ∗∗P < 0.01 versus the Con group; ##P < 0.01, #P < 0.05 versus the DM group; ▽▽P < 0.01, ▽P < 0.05 versus the ATV group. Values expressed as mean ± SD (n = 7 per group).
Fig 5: BMSCs inhibited pyroptosis of RTECs of rats with SI-AKI via the SITR1/Parkin axis. (A) Levels of inflammasome activation related-proteins (NLRP3, ASC and caspase-1) in RTECs of rats were detected using Western blotting; (B) Levels of inflammasome activation related-proteins (NLRP3, ASC and caspase-1) in RTECs of rats were detected using immunohistochemistry. (C) Survival rate of rats in each group. Each experiment was repeated for three times independently. Data are presented as mean ± standard deviation and analyzed using one-way ANOVA, followed by Tukey’s multiple comparison test for the post hoc test, * p < 0.05, ** p < 0.01, *** p < 0.001.
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