Fig 1: Changes in mRNA level of receptor tyrosine kinases in response to belinostat (BEL) and hydrazostat (HYD) measured by qRT-PCR. (a) KIT and FLT3 mRNA levels in leukemia cells incubated with DMSO, belinostat, or hydrazostat for 72 h. (b) PDGFRa and AXL mRNA levels in NB cells incubated with DMSO, belinostat, or hydrazostat for 72 h. (c) NTRK1, INSR, ROR2, and RET mRNA in leukemia HL-60, K562, Kasumi-1, THP-1, and NB Kelly, SH-SY5Y, LAN-1 cells incubated with belinostat or hydrazostat for 72 h. The color indicates a fold change of gene expression relative to control (DMSO). Fold changes higher than 3 are represented as bars in Supplementary File. (d) THP-1 cells were pretreated with DMSO, belinostat, or hydrazostat for 72 h and stained with an anti-FLT3 antibody (green) and DAPI dye (blue). K562 cells were pretreated with DMSO, belinostat, or hydrazostat for 72 h and stained with an anti-KIT antibody (green) and DAPI dye (blue). qRT-PCR was performed in three replicates. P-value was calculated by two-way ANOVA with no correction.